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Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)

Manganese toxicity is one of the growth limiting factors under acidic conditions. Some plants accumulate Mn more in the top than in the root, while others accumulate more in the root than in the top. Vegetable soybean is an indicator plant that can be used to determine the mechanism of toxicity....

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Autor principal: Djafruddin, Rosmaidar
Formato: Tese
Idioma:English
English
Publicado em: 2001
Assuntos:
Soybean - Case studies
Acesso em linha:http://psasir.upm.edu.my/id/eprint/10569/1/FP_2001_25.pdf
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spelling oai:psasir.upm.edu.my:10569 http://psasir.upm.edu.my/id/eprint/10569/ Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.) Djafruddin, Rosmaidar Manganese toxicity is one of the growth limiting factors under acidic conditions. Some plants accumulate Mn more in the top than in the root, while others accumulate more in the root than in the top. Vegetable soybean is an indicator plant that can be used to determine the mechanism of toxicity. Part of the plant tissue that accumulates the highest Mn needs to be extracted so as to determine the activity of superoxide dismutase (SOD). In view of this, a series of experiments was conducted to determine the accumulation of Mn in the tissues (leaf, stem, root and pod) of vegetable soybean, toxic level of Mn in solution culture, time-dependent of Mn accumulation in the tissue and the effect of Mn concentration on superoxide dismutase activity under different time of plant growth. Five experiments were conducted in glass house and laboratories at UPM. Experiment 1 was a sand culture experiment to determine the distribution of Mn accumulation in the plant tissues. Experiment 2 and 3 were solution culture experiments to observe toxic level of Mn. Experiment 4 was used to determine time-dependent of Mn accumulation, while experiment 5 was used to study the effect of Mn and time on superoxide dismutase activity. The results showed that the highest accumulation of Mn in vegetable soybean was in the leaves. The Mn concentration corresponding to 20% reduction in dry weight was 20 uM. Plant accumulated Mn more during vegetative stage compared to generative stage. Increasing the time of growth decreased the accumulation of Mn in the 5 and 25 uM of Mn treatments Degeneration of plant cell in toxic condition (25 uM) affected the growth of plant and inhibited nutrient movement from the root to the shoot. Enzyme extraction showed that the activity of superoxide dismutase increased up to 25.36 umol m L-1 of crude enzyme minute-1 at level 15 uM after 20 days of plant age in solution culture. 2001-09 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/10569/1/FP_2001_25.pdf Djafruddin, Rosmaidar (2001) Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.). Doctoral thesis, Universiti Putra Malaysia. Soybean - Case studies English
institution UPM IR
collection UPM IR
language English
English
topic Soybean - Case studies
spellingShingle Soybean - Case studies
Djafruddin, Rosmaidar
Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
description Manganese toxicity is one of the growth limiting factors under acidic conditions. Some plants accumulate Mn more in the top than in the root, while others accumulate more in the root than in the top. Vegetable soybean is an indicator plant that can be used to determine the mechanism of toxicity. Part of the plant tissue that accumulates the highest Mn needs to be extracted so as to determine the activity of superoxide dismutase (SOD). In view of this, a series of experiments was conducted to determine the accumulation of Mn in the tissues (leaf, stem, root and pod) of vegetable soybean, toxic level of Mn in solution culture, time-dependent of Mn accumulation in the tissue and the effect of Mn concentration on superoxide dismutase activity under different time of plant growth. Five experiments were conducted in glass house and laboratories at UPM. Experiment 1 was a sand culture experiment to determine the distribution of Mn accumulation in the plant tissues. Experiment 2 and 3 were solution culture experiments to observe toxic level of Mn. Experiment 4 was used to determine time-dependent of Mn accumulation, while experiment 5 was used to study the effect of Mn and time on superoxide dismutase activity. The results showed that the highest accumulation of Mn in vegetable soybean was in the leaves. The Mn concentration corresponding to 20% reduction in dry weight was 20 uM. Plant accumulated Mn more during vegetative stage compared to generative stage. Increasing the time of growth decreased the accumulation of Mn in the 5 and 25 uM of Mn treatments Degeneration of plant cell in toxic condition (25 uM) affected the growth of plant and inhibited nutrient movement from the root to the shoot. Enzyme extraction showed that the activity of superoxide dismutase increased up to 25.36 umol m L-1 of crude enzyme minute-1 at level 15 uM after 20 days of plant age in solution culture.
format Thesis
author Djafruddin, Rosmaidar
author_facet Djafruddin, Rosmaidar
author_sort Djafruddin, Rosmaidar
title Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_short Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_full Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_fullStr Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_full_unstemmed Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_sort manganese accumulation and toxicity, and superoxide dismutase activity in vegetable soybean (glycine max (l.) merr.)
publishDate 2001
url http://psasir.upm.edu.my/id/eprint/10569/1/FP_2001_25.pdf
_version_ 1819293478332399616
score 13.4562235

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