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Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia

Antimicrobial resistance is one of the major public health threats globally. This challenge has been aggravated with the overuse and misuse of antibiotics in food animals and humans. The present study aimed to investigate the prevalence of Extended-Spectrum β-lactamase (ESBL) genes in Escherichia co...

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Huvudupphovsmän: Lemlem, Mulu, Aklilu, Erkihun, Mohammed, Maizan, Kamaruzzaman, Fadhilah, Zakaria, Zunita, Harun, Azian, Devan, Susmita Seenu
Materialtyp: Artikel
Språk:English
Publicerad: Public Library of Science 2023
Länkar:http://psasir.upm.edu.my/id/eprint/108742/1/Molecular%20detection%20and%20antimicrobial%20resistance.pdf
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spelling oai:psasir.upm.edu.my:108742 http://psasir.upm.edu.my/id/eprint/108742/ Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia Lemlem, Mulu Aklilu, Erkihun Mohammed, Maizan Kamaruzzaman, Fadhilah Zakaria, Zunita Harun, Azian Devan, Susmita Seenu Antimicrobial resistance is one of the major public health threats globally. This challenge has been aggravated with the overuse and misuse of antibiotics in food animals and humans. The present study aimed to investigate the prevalence of Extended-Spectrum β-lactamase (ESBL) genes in Escherichia coli (E. coli) isolated from broiler chickens in Kelantan, Malaysia. A total of 320 cloacal swabs were collected from farms in different districts of Kelantan and were analyzed using routine bacteriology, antimicrobial susceptibility test, and molecular techniques for further identification and characterization of ESBL encoding genes. Based on PCR detection for the E. coli species-specific Pho gene, 30.3% (97/320) of isolates were confirmed as E. coli, and 84.5% (82/97) of the isolates were positive for at least one ESBL gene. Majority of the isolates, 62.9% (61/97) were harboring blaCTX-M followed by 45.4% (44/97) of blaTEM genes, while 16.5% (16/97) of the isolates were positive for both mcr-1 and ESBL genes. Overall, 93.8% (90/97) of the E. coli were resistant to three or more antimicrobials; indicating that the isolates were multi-drug resistance. 90.7% of multiple antibiotic resistance (MAR) index value greater than 0.2, would also suggest the isolates were from high-risk sources of contamination. The MLST result shows that the isolates are widely diverse. Our findings provide insight into the alarmingly high distribution of antimicrobial resistant bacteria, mainly ESBL producing E. coli in apparently healthy chickens indicating the role of food animals in the emergence and spread of antimicrobial resistance, and the potential public health threats it may pose. Public Library of Science 2023-05-19 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/108742/1/Molecular%20detection%20and%20antimicrobial%20resistance.pdf Lemlem, Mulu and Aklilu, Erkihun and Mohammed, Maizan and Kamaruzzaman, Fadhilah and Zakaria, Zunita and Harun, Azian and Devan, Susmita Seenu (2023) Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia. PLoS One, 18 (5). pp. 1-12. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0285743 10.1371/journal.pone.0285743
institution UPM IR
collection UPM IR
language English
description Antimicrobial resistance is one of the major public health threats globally. This challenge has been aggravated with the overuse and misuse of antibiotics in food animals and humans. The present study aimed to investigate the prevalence of Extended-Spectrum β-lactamase (ESBL) genes in Escherichia coli (E. coli) isolated from broiler chickens in Kelantan, Malaysia. A total of 320 cloacal swabs were collected from farms in different districts of Kelantan and were analyzed using routine bacteriology, antimicrobial susceptibility test, and molecular techniques for further identification and characterization of ESBL encoding genes. Based on PCR detection for the E. coli species-specific Pho gene, 30.3% (97/320) of isolates were confirmed as E. coli, and 84.5% (82/97) of the isolates were positive for at least one ESBL gene. Majority of the isolates, 62.9% (61/97) were harboring blaCTX-M followed by 45.4% (44/97) of blaTEM genes, while 16.5% (16/97) of the isolates were positive for both mcr-1 and ESBL genes. Overall, 93.8% (90/97) of the E. coli were resistant to three or more antimicrobials; indicating that the isolates were multi-drug resistance. 90.7% of multiple antibiotic resistance (MAR) index value greater than 0.2, would also suggest the isolates were from high-risk sources of contamination. The MLST result shows that the isolates are widely diverse. Our findings provide insight into the alarmingly high distribution of antimicrobial resistant bacteria, mainly ESBL producing E. coli in apparently healthy chickens indicating the role of food animals in the emergence and spread of antimicrobial resistance, and the potential public health threats it may pose.
format Article
author Lemlem, Mulu
Aklilu, Erkihun
Mohammed, Maizan
Kamaruzzaman, Fadhilah
Zakaria, Zunita
Harun, Azian
Devan, Susmita Seenu
spellingShingle Lemlem, Mulu
Aklilu, Erkihun
Mohammed, Maizan
Kamaruzzaman, Fadhilah
Zakaria, Zunita
Harun, Azian
Devan, Susmita Seenu
Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia
author_facet Lemlem, Mulu
Aklilu, Erkihun
Mohammed, Maizan
Kamaruzzaman, Fadhilah
Zakaria, Zunita
Harun, Azian
Devan, Susmita Seenu
author_sort Lemlem, Mulu
title Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia
title_short Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia
title_full Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia
title_fullStr Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia
title_full_unstemmed Molecular detection and antimicrobial resistance profiles of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli in broiler chicken farms in Malaysia
title_sort molecular detection and antimicrobial resistance profiles of extended-spectrum beta-lactamase (esbl) producing escherichia coli in broiler chicken farms in malaysia
publisher Public Library of Science
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/108742/1/Molecular%20detection%20and%20antimicrobial%20resistance.pdf
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score 13.4562235

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