Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-α2b in Escherichia coli
Two plasmids, pFLAG-ATS and pET 26b(+), were studied for the periplasmic expression of recombinant human interferon-α2b (IFN-α2b) in Escherichia coli. The pFLAG-ATS contains ompA signal sequence and tac promoter while pET 26b(+) contains pelB signal sequence and T7lac promoter. It was observed that...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11248/1/62.pdf |
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