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Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates

In non-human primates (NHPs), Mycobacterium avium complex (MAC) species are the major source of non-tuberculous mycobacteriosis, causing tuberculous-like lesions in lymph nodes and parenchymatous organs in zoo and wildlife animals. Poor species-specific detection by serological diagnosis has negativ...

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Päätekijät: Lekko, Yusuf Madaki, Che-Amat, Azlan, Ooi, Peck Toung, Omar, Sharina, Ramanoon, Siti Zubaidah, Mazlan, Mazlina, Abdullah Jesse, Faez Firdaus
Aineistotyyppi: Artikkeli
Kieli:English
Julkaistu: Universiti Putra Malaysia 2024
Linkit:http://psasir.upm.edu.my/id/eprint/113552/1/113552.pdf
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spelling oai:psasir.upm.edu.my:113552 http://psasir.upm.edu.my/id/eprint/113552/ Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates Lekko, Yusuf Madaki Che-Amat, Azlan Ooi, Peck Toung Omar, Sharina Ramanoon, Siti Zubaidah Mazlan, Mazlina Abdullah Jesse, Faez Firdaus In non-human primates (NHPs), Mycobacterium avium complex (MAC) species are the major source of non-tuberculous mycobacteriosis, causing tuberculous-like lesions in lymph nodes and parenchymatous organs in zoo and wildlife animals. Poor species-specific detection by serological diagnosis has negatively impacted the surveillance of MAC on non-human primates. Serum was collected from suspected twelve (n = 12) NHPs with no record of health monitoring, including gibbon (n = 5), capuchins (n = 2), siamang (n = 2), mandrill (n = 1), and orangutan (n = 2). An in-house avian purified protein derivative (aPPD) enzyme-linked immunosorbent assays (ELISA) antibody detection was developed and modified based on the established protocols. The aPPD ELISA for MAC antibodies detection at serum and Protein-G dilutions of 1:200-0.5µg/ml, respectively, detected 3/12 (25%) positive serum. At both serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, the aPPD ELISA detected 12/12 (100%), respectively. The antibody was not detected for an in-house aPPD ELISA with serum and anti-monkey immunoglobulin G (IgG) dilutions at 1:100-0.5 and 1:300-1 µg/ml. However, 2/12 (16%) was detected using serum and anti-monkey IgG dilutions at 1:200-0.05 µg/ml. An in-house aPPD ELISA procedure for MAC antibodies detection in primates, at serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, both have shown sensitivity and specificity of 100%, positive predictive value and negative predictive value of 100%, respectively. The serum and anti-monkey IgG have shown extremely low sensitivity and specificity. In conclusion, the performance of an in-house aPPD ELISA using three different dilutions on serum and conjugates in detecting MAC in a primate has shown that Protein-G horseradish peroxidase, as secondary conjugates were able to detect MAC antibodies. Universiti Putra Malaysia 2024 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/113552/1/113552.pdf Lekko, Yusuf Madaki and Che-Amat, Azlan and Ooi, Peck Toung and Omar, Sharina and Ramanoon, Siti Zubaidah and Mazlan, Mazlina and Abdullah Jesse, Faez Firdaus (2024) Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates. Pertanika Journal of Tropical Agricultural Science, 47 (2). pp. 549-557. ISSN 1511-3701; eISSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2911-2023 10.47836/pjtas.47.2.14
institution UPM IR
collection UPM IR
language English
description In non-human primates (NHPs), Mycobacterium avium complex (MAC) species are the major source of non-tuberculous mycobacteriosis, causing tuberculous-like lesions in lymph nodes and parenchymatous organs in zoo and wildlife animals. Poor species-specific detection by serological diagnosis has negatively impacted the surveillance of MAC on non-human primates. Serum was collected from suspected twelve (n = 12) NHPs with no record of health monitoring, including gibbon (n = 5), capuchins (n = 2), siamang (n = 2), mandrill (n = 1), and orangutan (n = 2). An in-house avian purified protein derivative (aPPD) enzyme-linked immunosorbent assays (ELISA) antibody detection was developed and modified based on the established protocols. The aPPD ELISA for MAC antibodies detection at serum and Protein-G dilutions of 1:200-0.5µg/ml, respectively, detected 3/12 (25%) positive serum. At both serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, the aPPD ELISA detected 12/12 (100%), respectively. The antibody was not detected for an in-house aPPD ELISA with serum and anti-monkey immunoglobulin G (IgG) dilutions at 1:100-0.5 and 1:300-1 µg/ml. However, 2/12 (16%) was detected using serum and anti-monkey IgG dilutions at 1:200-0.05 µg/ml. An in-house aPPD ELISA procedure for MAC antibodies detection in primates, at serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, both have shown sensitivity and specificity of 100%, positive predictive value and negative predictive value of 100%, respectively. The serum and anti-monkey IgG have shown extremely low sensitivity and specificity. In conclusion, the performance of an in-house aPPD ELISA using three different dilutions on serum and conjugates in detecting MAC in a primate has shown that Protein-G horseradish peroxidase, as secondary conjugates were able to detect MAC antibodies.
format Article
author Lekko, Yusuf Madaki
Che-Amat, Azlan
Ooi, Peck Toung
Omar, Sharina
Ramanoon, Siti Zubaidah
Mazlan, Mazlina
Abdullah Jesse, Faez Firdaus
spellingShingle Lekko, Yusuf Madaki
Che-Amat, Azlan
Ooi, Peck Toung
Omar, Sharina
Ramanoon, Siti Zubaidah
Mazlan, Mazlina
Abdullah Jesse, Faez Firdaus
Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
author_facet Lekko, Yusuf Madaki
Che-Amat, Azlan
Ooi, Peck Toung
Omar, Sharina
Ramanoon, Siti Zubaidah
Mazlan, Mazlina
Abdullah Jesse, Faez Firdaus
author_sort Lekko, Yusuf Madaki
title Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
title_short Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
title_full Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
title_fullStr Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
title_full_unstemmed Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
title_sort development of an in-house appd elisa for mycobacterium avium complex (mac) antibodies detection in zoo primates
publisher Universiti Putra Malaysia
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113552/1/113552.pdf
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score 13.4562235

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