A modified in situ RT-PCR method for localizing fungal-specific gene expression in Candida-infected mice renal cells

In situ Reverse Transcriptase PCR (in situ RT-PCR) can amplify mRNA and localize gene expression in cells. However, this method is not feasible in fungi as the thick fungal cell wall constitutes a barrier to this procedure. We developed a two step in situ RT-PCR procedure which enabled the detection...

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Bibliographic Details
Main Authors: Yong, Phelim Voon Chen, Ong, Khang Wei, Mohd Sidik, Shiran, Rosli, Rozita, Chong, Pei Pei
Format: Article
Published: Elsevier BV 2009
Online Access:http://psasir.upm.edu.my/id/eprint/12728/1/A%20modified%20in%20situ%20RT-PCR%20method%20for%20localizing%20fungal-specific%20gene%20expression%20in%20Candida-infected%20mice%20renal%20cells.pdf