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Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.

Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo...

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Main Authors: M.A., Bahmaninejad, Bejo, Mohd Hair, Omar, Abdul Rahman, Ideris, Aini, R., Toroghi
Format: Article
Language:English
English
Published: Academic Electronic Press Ltd. 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13553/1/Characterization%20of%20three%20infectious%20bursal%20disease%20virus%20isolates%20obtained%20from%20layer%20chickens%20in%20Iran.pdf
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spelling oai:psasir.upm.edu.my:13553 http://psasir.upm.edu.my/id/eprint/13553/ Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. M.A., Bahmaninejad Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini R., Toroghi Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo fibroblasts (CEFs) confirmed that the isolates were IBDVs. RT-PCR, restriction fragment length polymorphism (RFLP), and phylogenetic analysis demonstrated that the isolates were very virulent IBDV (vvIBDV) and showed a nucleotide sequence similarity of 96.3 to 99.8% in comparison with other vvIBDV strains. It was concluded that the Iranian isolates represented vvIBDV of serotype 1 originating from Europe, Japan, and China. Academic Electronic Press Ltd. 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13553/1/Characterization%20of%20three%20infectious%20bursal%20disease%20virus%20isolates%20obtained%20from%20layer%20chickens%20in%20Iran.pdf M.A., Bahmaninejad and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and R., Toroghi (2008) Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. Acta Virologica, 52 (3). pp. 167-174. ISSN 0001-723X English
institution UPM IR
collection UPM IR
language English
English
description Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo fibroblasts (CEFs) confirmed that the isolates were IBDVs. RT-PCR, restriction fragment length polymorphism (RFLP), and phylogenetic analysis demonstrated that the isolates were very virulent IBDV (vvIBDV) and showed a nucleotide sequence similarity of 96.3 to 99.8% in comparison with other vvIBDV strains. It was concluded that the Iranian isolates represented vvIBDV of serotype 1 originating from Europe, Japan, and China.
format Article
author M.A., Bahmaninejad
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
R., Toroghi
spellingShingle M.A., Bahmaninejad
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
R., Toroghi
Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
author_facet M.A., Bahmaninejad
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
R., Toroghi
author_sort M.A., Bahmaninejad
title Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
title_short Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
title_full Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
title_fullStr Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
title_full_unstemmed Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
title_sort characterization of three infectious bursal disease virus isolates obtained from layer chickens in iran.
publisher Academic Electronic Press Ltd.
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/13553/1/Characterization%20of%20three%20infectious%20bursal%20disease%20virus%20isolates%20obtained%20from%20layer%20chickens%20in%20Iran.pdf
_version_ 1819293721905070080
score 13.4562235

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