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Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.

Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunoso...

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Autori principali: B., Chaharaein, Omar, Abdul Rahman, Ideris, Aini, Yusoff, Khatijah, Syed Hassan, Sharifah
Natura: Articolo
Lingua:English
English
Pubblicazione: Elsevier 2009
Accesso online:http://psasir.upm.edu.my/id/eprint/13933/1/Detection%20of%20H5.pdf
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spelling oai:psasir.upm.edu.my:13933 http://psasir.upm.edu.my/id/eprint/13933/ Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. B., Chaharaein Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Syed Hassan, Sharifah Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR–ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13933/1/Detection%20of%20H5.pdf B., Chaharaein and Omar, Abdul Rahman and Ideris, Aini and Yusoff, Khatijah and Syed Hassan, Sharifah (2009) Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. Microbiological Research, 164 (2). pp. 174-179. ISSN 0944-5013 10.1016/j.micres.2007.01.001 English
institution UPM IR
collection UPM IR
language English
English
description Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR–ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes.
format Article
author B., Chaharaein
Omar, Abdul Rahman
Ideris, Aini
Yusoff, Khatijah
Syed Hassan, Sharifah
spellingShingle B., Chaharaein
Omar, Abdul Rahman
Ideris, Aini
Yusoff, Khatijah
Syed Hassan, Sharifah
Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
author_facet B., Chaharaein
Omar, Abdul Rahman
Ideris, Aini
Yusoff, Khatijah
Syed Hassan, Sharifah
author_sort B., Chaharaein
title Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
title_short Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
title_full Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
title_fullStr Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
title_full_unstemmed Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
title_sort detection of h5, h7 and h9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
publisher Elsevier
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/13933/1/Detection%20of%20H5.pdf
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score 13.4562235

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