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Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus

A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 deg...

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Autores Principales:	Abd. Shukor , Mohd. Yunus, Rahman, MF, Suhaili, Zarizal, Shamaan, Nor Aripin, Syed, Mohd. Arif
Formato:	Artículo
Lenguaje:	English English
Publicado:	Elsevier B.V. 2010
Materias:	Molybdenum - Analysis Acinetobacter
Acceso en línea:	http://psasir.upm.edu.my/id/eprint/14999/1/Hexavalent%20molybdenum%20reduction%20to%20mo.pdf
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record_format	eprints
spelling	oai:psasir.upm.edu.my:14999 http://psasir.upm.edu.my/id/eprint/14999/ Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. Elsevier B.V. 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14999/1/Hexavalent%20molybdenum%20reduction%20to%20mo.pdf Abd. Shukor , Mohd. Yunus and Rahman, MF and Suhaili, Zarizal and Shamaan, Nor Aripin and Syed, Mohd. Arif (2010) Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus. Folia microbiologica, 55 (2). pp. 137-143. ISSN 0015-5632 Molybdenum - Analysis Acinetobacter DOI: 10.1007/s12223-010-0021-x English
institution	UPM IR
collection	UPM IR
language	English English
topic	Molybdenum - Analysis Acinetobacter
spellingShingle	Molybdenum - Analysis Acinetobacter Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
description	A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction.
format	Article
author	Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif
author_facet	Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif
author_sort	Abd. Shukor , Mohd. Yunus
title	Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
title_short	Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
title_full	Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
title_fullStr	Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
title_full_unstemmed	Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
title_sort	hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
publisher	Elsevier B.V.
publishDate	2010
url	http://psasir.upm.edu.my/id/eprint/14999/1/Hexavalent%20molybdenum%20reduction%20to%20mo.pdf
_version_	1819293874897551360
score	13.4562235

Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus

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