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Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology

Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folat...

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গ্রন্থ-পঞ্জীর বিবরন
প্রধান লেখক: Muhamad Nor, Norfarina, Mohamad, Rosfarizan, Foo, Hooi Ling, Abdul Rahim, Raha
বিন্যাস: প্রবন্ধ
ভাষা:English
প্রকাশিত: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia 2010
অনলাইন ব্যবহার করুন:http://psasir.upm.edu.my/id/eprint/15142/1/Improvement%20of%20folate%20biosynthesis%20by%20lactic%20acid%20bacteria%20using%20response%20surface%20methodology.pdf
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id oai:psasir.upm.edu.my:15142
record_format eprints
spelling oai:psasir.upm.edu.my:15142 http://psasir.upm.edu.my/id/eprint/15142/ Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology Muhamad Nor, Norfarina Mohamad, Rosfarizan Foo, Hooi Ling Abdul Rahim, Raha Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM. Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15142/1/Improvement%20of%20folate%20biosynthesis%20by%20lactic%20acid%20bacteria%20using%20response%20surface%20methodology.pdf Muhamad Nor, Norfarina and Mohamad, Rosfarizan and Foo, Hooi Ling and Abdul Rahim, Raha (2010) Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology. Food Technology and Biotechnology, 48 (2). pp. 243-250. ISSN 1330-9862; ESSN: 1334-2606 http://www.ftb.com.hr/index.php/archives/61-volume-48-issue-no-2/165
institution UPM IR
collection UPM IR
language English
description Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM.
format Article
author Muhamad Nor, Norfarina
Mohamad, Rosfarizan
Foo, Hooi Ling
Abdul Rahim, Raha
spellingShingle Muhamad Nor, Norfarina
Mohamad, Rosfarizan
Foo, Hooi Ling
Abdul Rahim, Raha
Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
author_facet Muhamad Nor, Norfarina
Mohamad, Rosfarizan
Foo, Hooi Ling
Abdul Rahim, Raha
author_sort Muhamad Nor, Norfarina
title Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
title_short Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
title_full Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
title_fullStr Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
title_full_unstemmed Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
title_sort improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
publisher Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/15142/1/Improvement%20of%20folate%20biosynthesis%20by%20lactic%20acid%20bacteria%20using%20response%20surface%20methodology.pdf
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score 13.4562235

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