Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate,...
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| Principais autores: | , , , , , , |
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| Formato: | Atigo |
| Idioma: | English |
| Publicado em: |
Triveni Enterprises
2009
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| Acesso em linha: | http://psasir.upm.edu.my/id/eprint/15320/1/Isolation%20and%20characterization%20of%20a%20Pseudomonas%20diesel.pdf |
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| Resumo: | A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium. |
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