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Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.

Accumulating data demonstrated that hypercholesterolemia and oxidative stress play an important role in the development of atherosclerosis. In the present study, a protective activity of alpha-lipoic acid; a metabolic antioxidant in hypercholesterolemic-induced animals was investigated. Eighteen adu...

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Prif Awduron: Amom, Zulkhairi, Zakaria, Zaiton, Mohamed, Jamaludin, Azlan, Azrina, Bahari, Hasnah, Baharuldin, Mohamad Taufik Hidayat, Mohd Moklas, Mohamad Aris, Osman, Khairul, Asmawi, Zanariyah, Nik Hassan, Mohamed Kamal
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Iaith:English
English
Cyhoeddwyd: Institute of Applied Biochemistry 2008
Mynediad Ar-lein:http://psasir.upm.edu.my/id/eprint/15464/1/Lipid%20lowering%20effect%20of%20antioxidant%20alpha.pdf
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id oai:psasir.upm.edu.my:15464
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spelling oai:psasir.upm.edu.my:15464 http://psasir.upm.edu.my/id/eprint/15464/ Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis. Amom, Zulkhairi Zakaria, Zaiton Mohamed, Jamaludin Azlan, Azrina Bahari, Hasnah Baharuldin, Mohamad Taufik Hidayat Mohd Moklas, Mohamad Aris Osman, Khairul Asmawi, Zanariyah Nik Hassan, Mohamed Kamal Accumulating data demonstrated that hypercholesterolemia and oxidative stress play an important role in the development of atherosclerosis. In the present study, a protective activity of alpha-lipoic acid; a metabolic antioxidant in hypercholesterolemic-induced animals was investigated. Eighteen adult male New Zealand White (NZW) rabbit were segregated into three groups labelled as group N, HCD and ALA (n = 6). Group N (normal control) was fed with normal chow, the rest (HCD and ALA) were fed with 100 g/head/day of 1% cholesterol rich diet to induce hypercholesterolemia. Four point two mg/body weight of alpha lipoic acid was concomintantly supplemented to the ALA group. Drinking water was given ad-libitum. The study was designed for 10 weeks. Blood sampling was taken from the ear lobe vein at the beginning, week 5 and week 10. Plasma was prepared for lipid profile estimation and microsomal lipid peroxidation index indicated with malondialdehyde (MDA) formation. At the end of the experiment, the animals were sacrificed and the aorta were excised for intimal lesion analysis. The plasma total cholesterol (TC) and low density lipoprotein (LDL) levels were found to be significantly low in ALA group compared to that of the HCD group (P<0.05). Similarly, low level of MDA (p<0.05) in ALA group was observed compared to that of the HCD group showing a significant reduction of lipid peroxidation activity. Histomorphometric intimal lesion analysis of the aorta showing less of atheromatous plaque formation in alpha lipoic acid supplemented group (p<0.05) compared to HCD group. These findings suggested that alpha lipoic acid posses a dual lipid lowering and anti-atherosclerotic properties indicated with low plasma TC and LDL levels and reduction of athero-lesion formation in hypercholesterolemic-induced rabbits. Institute of Applied Biochemistry 2008-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15464/1/Lipid%20lowering%20effect%20of%20antioxidant%20alpha.pdf Amom, Zulkhairi and Zakaria, Zaiton and Mohamed, Jamaludin and Azlan, Azrina and Bahari, Hasnah and Baharuldin, Mohamad Taufik Hidayat and Mohd Moklas, Mohamad Aris and Osman, Khairul and Asmawi, Zanariyah and Nik Hassan, Mohamed Kamal (2008) Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis. Journal of Clinical Biochemistry and Nutrition, 43 (2). pp. 88-94. ISSN 0912-0009, ESSN: 1880-5086 http://www.agbi.tsukuba.ac.jp 10.3164/jcbn.2008051 English
institution UPM IR
collection UPM IR
language English
English
description Accumulating data demonstrated that hypercholesterolemia and oxidative stress play an important role in the development of atherosclerosis. In the present study, a protective activity of alpha-lipoic acid; a metabolic antioxidant in hypercholesterolemic-induced animals was investigated. Eighteen adult male New Zealand White (NZW) rabbit were segregated into three groups labelled as group N, HCD and ALA (n = 6). Group N (normal control) was fed with normal chow, the rest (HCD and ALA) were fed with 100 g/head/day of 1% cholesterol rich diet to induce hypercholesterolemia. Four point two mg/body weight of alpha lipoic acid was concomintantly supplemented to the ALA group. Drinking water was given ad-libitum. The study was designed for 10 weeks. Blood sampling was taken from the ear lobe vein at the beginning, week 5 and week 10. Plasma was prepared for lipid profile estimation and microsomal lipid peroxidation index indicated with malondialdehyde (MDA) formation. At the end of the experiment, the animals were sacrificed and the aorta were excised for intimal lesion analysis. The plasma total cholesterol (TC) and low density lipoprotein (LDL) levels were found to be significantly low in ALA group compared to that of the HCD group (P<0.05). Similarly, low level of MDA (p<0.05) in ALA group was observed compared to that of the HCD group showing a significant reduction of lipid peroxidation activity. Histomorphometric intimal lesion analysis of the aorta showing less of atheromatous plaque formation in alpha lipoic acid supplemented group (p<0.05) compared to HCD group. These findings suggested that alpha lipoic acid posses a dual lipid lowering and anti-atherosclerotic properties indicated with low plasma TC and LDL levels and reduction of athero-lesion formation in hypercholesterolemic-induced rabbits.
format Article
author Amom, Zulkhairi
Zakaria, Zaiton
Mohamed, Jamaludin
Azlan, Azrina
Bahari, Hasnah
Baharuldin, Mohamad Taufik Hidayat
Mohd Moklas, Mohamad Aris
Osman, Khairul
Asmawi, Zanariyah
Nik Hassan, Mohamed Kamal
spellingShingle Amom, Zulkhairi
Zakaria, Zaiton
Mohamed, Jamaludin
Azlan, Azrina
Bahari, Hasnah
Baharuldin, Mohamad Taufik Hidayat
Mohd Moklas, Mohamad Aris
Osman, Khairul
Asmawi, Zanariyah
Nik Hassan, Mohamed Kamal
Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
author_facet Amom, Zulkhairi
Zakaria, Zaiton
Mohamed, Jamaludin
Azlan, Azrina
Bahari, Hasnah
Baharuldin, Mohamad Taufik Hidayat
Mohd Moklas, Mohamad Aris
Osman, Khairul
Asmawi, Zanariyah
Nik Hassan, Mohamed Kamal
author_sort Amom, Zulkhairi
title Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
title_short Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
title_full Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
title_fullStr Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
title_full_unstemmed Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
title_sort lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis.
publisher Institute of Applied Biochemistry
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/15464/1/Lipid%20lowering%20effect%20of%20antioxidant%20alpha.pdf
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score 13.4562235

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