TY  - JOUR
T1  - Optimization of biolistic bombardment parameters for Dendrobium sonia 17 calluses using GFP and GUS as the reporter system
A1  - Tee, Chong Siang
LA  - English
LA  - English
PB  - Springer
YR  - 2005
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:18372
AB  - Genetic transformation system of Dendrobium Sonia 17 was optimized using green fluorescent protein (GFP) and β-glucuronidase (GUS) gene as the reporter systems. The 35S-sgfp-TYG-nos (p35S) and pSMDFR, carrying sgfp and gusA gene, respectively, were co-bombarded into the calluses. Parameters optimized were acceleration pressure, target distance, gold particle size, pre-bombardment cultured time, plasmid DNA precipitation, total plasmid DNA and the ratio of the plasmids co-bombarded. Both reporter systems responded similarly to the bombardment parameters investigated. Based on the GUS/GFP spot counts, the GFP expression rate was higher than that for GUS under the control of the same promoter, CaMV 35S. GFP could be used as the reporter system for the co-bombardment as it was rapid and non-destructive system to monitor the transformed tissues. A combination of GFP and antibiotic resistance gene was used to select stable putative transformants. © Springer 2005.
KW  - Plant genetic transformation
KW  - Dendrobium - Genetics
KW  - Green fluorescent protein
ER  -