Optimization of a native gel electrophoretic process for the purification of intracellular green fluorescent protein from intact Escherichia coli cells
Intracellular green fluorescent protein (GFP) can be separated and purified from intact Escherichia coli cells by a preparative native polyacrylamide gel electrophoresis (n-PAGE). The effects of operating parameters such as the volume and concentration of feedstock and the pore size and height of re...
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| Main Authors: | , , , |
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| Format: | Article |
| Sprog: | English |
| Udgivet: |
Elsevier
2011
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| Online adgang: | http://psasir.upm.edu.my/id/eprint/23105/1/Optimization%20of%20a%20native%20gel%20electrophoretic%20process%20for%20the%20purification%20of%20intracellular%20green%20fluorescent%20protein%20from%20intact%20Escherichia%20coli%20cells.pdf |
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