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Molecular and pathological identification of feline coronavirus type I

The coronavirus in cats has been described as feline infectious peritonitis (FIPV) and feline enteric coronavirus (FECV). FIPV is highly fatal and caused immune-mediated pyogranulomatous disease, whereas FECV causes mild enteric infection. In this study, we described the isolation and molecular char...

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Hauptverfasser: Amer, Alazawy, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman, Tengku Ibrahim, Tengku Azmi, Bande, Faruku, Ajwad, Assumaidaee
Format: Artikel
Sprache:English
Veröffentlicht: Academic Journals 2012
Online Zugang:http://psasir.upm.edu.my/id/eprint/28392/1/28392.pdf
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spelling oai:psasir.upm.edu.my:28392 http://psasir.upm.edu.my/id/eprint/28392/ Molecular and pathological identification of feline coronavirus type I Amer, Alazawy Arshad, Siti Suri Bejo, Mohd Hair Omar, Abdul Rahman Tengku Ibrahim, Tengku Azmi Bande, Faruku Ajwad, Assumaidaee The coronavirus in cats has been described as feline infectious peritonitis (FIPV) and feline enteric coronavirus (FECV). FIPV is highly fatal and caused immune-mediated pyogranulomatous disease, whereas FECV causes mild enteric infection. In this study, we described the isolation and molecular characterization of naturally occurring feline coronavirus from domestic cat in Malaysia. Additionally, the resultant pathological conditions observed in the infected cat were reported. Ascitic fluid sample was collected from 3-year-old domestic cat clinically suspected of effusive (wet form) FIP and subjected to virus isolation in Felis catus whole fetus cell cultures (Fcwf-4). The result of virus isolation was confirmed using one step reverse transcription polymerase chain reaction (RT-PCR). To gain insight on the genetic variant of FCoV, the S-gene sequence was amplified using type 1 specific primers.Virus isolation showed cytopathic effect (CPE) characterized by giant cells, ballooning and cells detachment. Ultrastructural findings showed virus particles attached to plasma membrane and later invaginated from the cell membrane. Virus-like particles were also observed in the vacuoles, likely as a result of spillage of mature virus-like particles into the cytoplasmic matrix. Histopathological examination of kidney, spleen and intestine organ samples revealed coagulative necrosis with infiltration of neutrophils and mononuclear cells. In conclusion, this study reports the first isolation of feline coronavirus in Malaysian cats and importantly the isolated virus was confirmed to be type I using S-gene amplification. Academic Journals 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/28392/1/28392.pdf Amer, Alazawy and Arshad, Siti Suri and Bejo, Mohd Hair and Omar, Abdul Rahman and Tengku Ibrahim, Tengku Azmi and Bande, Faruku and Ajwad, Assumaidaee (2012) Molecular and pathological identification of feline coronavirus type I. African Journal of Biotechnology, 11 (45). art. no. D940FC031967. pp. 10451-10461. ISSN 1684-5315 https://academicjournals.org/journal/AJB/article-abstract/D940FC031967 10.5897/AJB11.3827
institution UPM IR
collection UPM IR
language English
description The coronavirus in cats has been described as feline infectious peritonitis (FIPV) and feline enteric coronavirus (FECV). FIPV is highly fatal and caused immune-mediated pyogranulomatous disease, whereas FECV causes mild enteric infection. In this study, we described the isolation and molecular characterization of naturally occurring feline coronavirus from domestic cat in Malaysia. Additionally, the resultant pathological conditions observed in the infected cat were reported. Ascitic fluid sample was collected from 3-year-old domestic cat clinically suspected of effusive (wet form) FIP and subjected to virus isolation in Felis catus whole fetus cell cultures (Fcwf-4). The result of virus isolation was confirmed using one step reverse transcription polymerase chain reaction (RT-PCR). To gain insight on the genetic variant of FCoV, the S-gene sequence was amplified using type 1 specific primers.Virus isolation showed cytopathic effect (CPE) characterized by giant cells, ballooning and cells detachment. Ultrastructural findings showed virus particles attached to plasma membrane and later invaginated from the cell membrane. Virus-like particles were also observed in the vacuoles, likely as a result of spillage of mature virus-like particles into the cytoplasmic matrix. Histopathological examination of kidney, spleen and intestine organ samples revealed coagulative necrosis with infiltration of neutrophils and mononuclear cells. In conclusion, this study reports the first isolation of feline coronavirus in Malaysian cats and importantly the isolated virus was confirmed to be type I using S-gene amplification.
format Article
author Amer, Alazawy
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Tengku Ibrahim, Tengku Azmi
Bande, Faruku
Ajwad, Assumaidaee
spellingShingle Amer, Alazawy
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Tengku Ibrahim, Tengku Azmi
Bande, Faruku
Ajwad, Assumaidaee
Molecular and pathological identification of feline coronavirus type I
author_facet Amer, Alazawy
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Tengku Ibrahim, Tengku Azmi
Bande, Faruku
Ajwad, Assumaidaee
author_sort Amer, Alazawy
title Molecular and pathological identification of feline coronavirus type I
title_short Molecular and pathological identification of feline coronavirus type I
title_full Molecular and pathological identification of feline coronavirus type I
title_fullStr Molecular and pathological identification of feline coronavirus type I
title_full_unstemmed Molecular and pathological identification of feline coronavirus type I
title_sort molecular and pathological identification of feline coronavirus type i
publisher Academic Journals
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/28392/1/28392.pdf
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score 13.4562235

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