Vista Equipo: Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation

Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation

The aim of present study was to detect the presence of C. pseudotuberculosis in the brain of the mice following oral inoculation as a model using PCR. Caseous lymphadenitis is a chronic and subclinical disease of sheep and goats which has universal distributions, presenting enormous animal and flock...

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Autores Principales:	Abdullah, Faez Firdaus Jesse, Adamu, Lawan, Osman, Abdinasir Yusuf, Muhdi, Ahmad Fauzan, Haron, Abdul Wahid, Saharee, Abdul Aziz, Saad, Mohd Zamri, Omar, Abdul Rahman
Formato:	Artículo
Lenguaje:	English
Publicado:	Maxwell Science Organization 2013
Acceso en línea:	http://psasir.upm.edu.my/id/eprint/29843/1/Polymerase%20chain%20reaction%20detection%20of%20C.pdf
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spelling	oai:psasir.upm.edu.my:29843 http://psasir.upm.edu.my/id/eprint/29843/ Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation Abdullah, Faez Firdaus Jesse Adamu, Lawan Osman, Abdinasir Yusuf Muhdi, Ahmad Fauzan Haron, Abdul Wahid Saharee, Abdul Aziz Saad, Mohd Zamri Omar, Abdul Rahman The aim of present study was to detect the presence of C. pseudotuberculosis in the brain of the mice following oral inoculation as a model using PCR. Caseous lymphadenitis is a chronic and subclinical disease of sheep and goats which has universal distributions, presenting enormous animal and flock prevalence. Total of 16 mice were used for this study, 8 mice were inoculated orally with 1.0 mL sterile phosphate buffered saline pH 7, while another 8 mice were inoculated with 1.0 mL of 109 colony forming unit of C. pseudotuberculosis. Seven different organs were collected during post mortem for the detection of C. pseudotuberculosis The result indicated 3 positive samples in lymph nodes, 5 in the brain and 1 in the liver. The PCR used in the present study may successfully be applied for the detection and diagnosis of C. pseudotuberculosis in the brain of the mice following oral inoculation. Maxwell Science Organization 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29843/1/Polymerase%20chain%20reaction%20detection%20of%20C.pdf Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Osman, Abdinasir Yusuf and Muhdi, Ahmad Fauzan and Haron, Abdul Wahid and Saharee, Abdul Aziz and Saad, Mohd Zamri and Omar, Abdul Rahman (2013) Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation. International Journal of Animal and Veterinary Advances, 5 (1). pp. 29-33. ISSN 2041-2894; ESSN: 2041-2908 http://maxwellsci.com/jp/abstract.php?jid=IJAVA&no=282&abs=05
institution	UPM IR
collection	UPM IR
language	English
description	The aim of present study was to detect the presence of C. pseudotuberculosis in the brain of the mice following oral inoculation as a model using PCR. Caseous lymphadenitis is a chronic and subclinical disease of sheep and goats which has universal distributions, presenting enormous animal and flock prevalence. Total of 16 mice were used for this study, 8 mice were inoculated orally with 1.0 mL sterile phosphate buffered saline pH 7, while another 8 mice were inoculated with 1.0 mL of 109 colony forming unit of C. pseudotuberculosis. Seven different organs were collected during post mortem for the detection of C. pseudotuberculosis The result indicated 3 positive samples in lymph nodes, 5 in the brain and 1 in the liver. The PCR used in the present study may successfully be applied for the detection and diagnosis of C. pseudotuberculosis in the brain of the mice following oral inoculation.
format	Article
author	Abdullah, Faez Firdaus Jesse Adamu, Lawan Osman, Abdinasir Yusuf Muhdi, Ahmad Fauzan Haron, Abdul Wahid Saharee, Abdul Aziz Saad, Mohd Zamri Omar, Abdul Rahman
spellingShingle	Abdullah, Faez Firdaus Jesse Adamu, Lawan Osman, Abdinasir Yusuf Muhdi, Ahmad Fauzan Haron, Abdul Wahid Saharee, Abdul Aziz Saad, Mohd Zamri Omar, Abdul Rahman Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
author_facet	Abdullah, Faez Firdaus Jesse Adamu, Lawan Osman, Abdinasir Yusuf Muhdi, Ahmad Fauzan Haron, Abdul Wahid Saharee, Abdul Aziz Saad, Mohd Zamri Omar, Abdul Rahman
author_sort	Abdullah, Faez Firdaus Jesse
title	Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
title_short	Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
title_full	Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
title_fullStr	Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
title_full_unstemmed	Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
title_sort	polymerase chain reaction detection of c. pseudotuberculosis in the brain of mice following oral inoculation
publisher	Maxwell Science Organization
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/29843/1/Polymerase%20chain%20reaction%20detection%20of%20C.pdf
_version_	1819295265275772928
score	13.4562235

Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation

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