Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia

A complete nucleotide (nt) coding sequence of infectious bursal disease virus (IBDV) isolate of Malaysia (UPM04190) was determined. In segment A, 3260 nt was identified, consisting of 2 overlapping open reading frame, predicted to be the VP5 of 465 nt with 154 amino acids and the polyprotein of 3039...

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প্রধান লেখক: Liew, Pit Sze, Omar, Abdul Rahman, Ideris, Aini, Bejo, Mohd Hair
বিন্যাস: Conference or Workshop Item
ভাষা:English
প্রকাশিত: Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013
অনলাইন ব্যবহার করুন:http://psasir.upm.edu.my/id/eprint/41398/1/41398.pdf
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spelling oai:psasir.upm.edu.my:41398 http://psasir.upm.edu.my/id/eprint/41398/ Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia Liew, Pit Sze Omar, Abdul Rahman Ideris, Aini Bejo, Mohd Hair A complete nucleotide (nt) coding sequence of infectious bursal disease virus (IBDV) isolate of Malaysia (UPM04190) was determined. In segment A, 3260 nt was identified, consisting of 2 overlapping open reading frame, predicted to be the VP5 of 465 nt with 154 amino acids and the polyprotein of 3039 nt with 1012 amino acids. In segment B, 2825 nt was obtained and consisted one open reading frame with 2640 nt predicted to be the 879 amino acids of VP1. Comparison of the deduced amino acids revealed 8 unique amino acids which were conserved only in UPM04190. Of all these amino acids, 3 were found in VP1, 3 were in VP2, and 2 were in VP5. The overall branching pattern of the phylogenetic trees revealed a similar pattern. Taken together, nt sequence and phylogenetic analyses revealed and suggested that our local isolate UPM04190 share a high homology with the very virulent strains. Analysis of the IBDV nt and amino acid sequence are best targeted at both genomes. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41398/1/41398.pdf Liew, Pit Sze and Omar, Abdul Rahman and Ideris, Aini and Bejo, Mohd Hair (2013) Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 99-100). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf
institution UPM IR
collection UPM IR
language English
description A complete nucleotide (nt) coding sequence of infectious bursal disease virus (IBDV) isolate of Malaysia (UPM04190) was determined. In segment A, 3260 nt was identified, consisting of 2 overlapping open reading frame, predicted to be the VP5 of 465 nt with 154 amino acids and the polyprotein of 3039 nt with 1012 amino acids. In segment B, 2825 nt was obtained and consisted one open reading frame with 2640 nt predicted to be the 879 amino acids of VP1. Comparison of the deduced amino acids revealed 8 unique amino acids which were conserved only in UPM04190. Of all these amino acids, 3 were found in VP1, 3 were in VP2, and 2 were in VP5. The overall branching pattern of the phylogenetic trees revealed a similar pattern. Taken together, nt sequence and phylogenetic analyses revealed and suggested that our local isolate UPM04190 share a high homology with the very virulent strains. Analysis of the IBDV nt and amino acid sequence are best targeted at both genomes.
format Conference or Workshop Item
author Liew, Pit Sze
Omar, Abdul Rahman
Ideris, Aini
Bejo, Mohd Hair
spellingShingle Liew, Pit Sze
Omar, Abdul Rahman
Ideris, Aini
Bejo, Mohd Hair
Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
author_facet Liew, Pit Sze
Omar, Abdul Rahman
Ideris, Aini
Bejo, Mohd Hair
author_sort Liew, Pit Sze
title Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
title_short Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
title_full Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
title_fullStr Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
title_full_unstemmed Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
title_sort complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of malaysia
publisher Faculty of Veterinary Medicine, Universiti Putra Malaysia
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/41398/1/41398.pdf
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score 13.4562235