TY  - JOUR
T1  - Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
A1  - Ramanujam, Priadarishni
LA  - English
PB  - Veterinary Association Malaysia
YR  - 2004
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:41473
AB  - The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its  virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The Fa cleavage is a prerequisite for producing infectious particles
whereby the nature of the cleavage site correlates with virulence of the virus. Inthis study we determined the F protein gene cleavage site nucleotide sequence ofNDV local isolates 2641191 P2, 5953/89 P3, 1266/89 P3 and 8820/92 P3. Their sequences were also compared with other NDV strains and isolates, such as AF2240, OOIIKS, 011TM, 01lC, 3410/92 P2, 6385/90 P3, V4 Que and F that had previously been determined. The deduced amino acid sequence of this site revealed the pathogenecity of these strains whereby 2641191 P2 and 5953/89 P3 were classified as virulent isolates whilst 1266/89 P3 and 8820/92 P3 were avirulent isolates.

ER  -