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Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)

This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed wit...

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Main Authors: Uda, Muhammad Nur Aiman, Che Man, Hasfalina, Abd Aziz, Samsuzana, Ishak, Zamri, Bunawan, Siti Noraini, Wahid, Nur Sabrina, Hashim, Uda
Formato: Artigo
Idioma:English
Publicado em: Elsevier 2014
Acesso em linha:http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf
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id oai:psasir.upm.edu.my:43204
record_format eprints
spelling oai:psasir.upm.edu.my:43204 http://psasir.upm.edu.my/id/eprint/43204/ Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV) Uda, Muhammad Nur Aiman Che Man, Hasfalina Abd Aziz, Samsuzana Ishak, Zamri Bunawan, Siti Noraini Wahid, Nur Sabrina Hashim, Uda This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively. Elsevier 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf Uda, Muhammad Nur Aiman and Che Man, Hasfalina and Abd Aziz, Samsuzana and Ishak, Zamri and Bunawan, Siti Noraini and Wahid, Nur Sabrina and Hashim, Uda (2014) Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV). Agriculture and Agricultural Science Procedia, 2. pp. 107-112. ISSN 2210-7843 http://www.sciencedirect.com/science/article/pii/S2210784314000175 10.1016/j.aaspro.2014.11.016
institution UPM IR
collection UPM IR
language English
description This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively.
format Article
author Uda, Muhammad Nur Aiman
Che Man, Hasfalina
Abd Aziz, Samsuzana
Ishak, Zamri
Bunawan, Siti Noraini
Wahid, Nur Sabrina
Hashim, Uda
spellingShingle Uda, Muhammad Nur Aiman
Che Man, Hasfalina
Abd Aziz, Samsuzana
Ishak, Zamri
Bunawan, Siti Noraini
Wahid, Nur Sabrina
Hashim, Uda
Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
author_facet Uda, Muhammad Nur Aiman
Che Man, Hasfalina
Abd Aziz, Samsuzana
Ishak, Zamri
Bunawan, Siti Noraini
Wahid, Nur Sabrina
Hashim, Uda
author_sort Uda, Muhammad Nur Aiman
title Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
title_short Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
title_full Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
title_fullStr Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
title_full_unstemmed Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
title_sort comparison study of two different isolation and purification method for rice tungro bacilliform virus (rtbv)
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf
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score 13.4562235

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