TY  - JOUR
T1  - Comparison study of two different isolation and purification
method for rice tungro bacilliform virus (RTBV)
A1  - Uda, Muhammad Nur Aiman
LA  - English
PB  - Elsevier
YR  - 2014
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:43204
AB  - This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively.
ER  -