Copertina

Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus

The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow r...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Sabullah, Mohd Khalizan, Abd. Shukor, Mohd Yunus, Shamaan, Nor Aripin, Khalid, Ariff, Ganzau, Azlan Jualang, Sulaiman, Mohd Rosni, Jirangon, Hussain, Ahmad, Siti Aqlima
Natura: Articolo
Lingua:English
Pubblicazione: Friends Science Publishers 2015
Accesso online:http://psasir.upm.edu.my/id/eprint/46049/1/Purification%20and%20anticholinesterase%20sensitivity%20of%20cholinesterase%20extracted%20from%20liver%20tissue%20of%20Puntius%20javanicus.pdf
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !
id oai:psasir.upm.edu.my:46049
record_format eprints
spelling oai:psasir.upm.edu.my:46049 http://psasir.upm.edu.my/id/eprint/46049/ Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus Sabullah, Mohd Khalizan Abd. Shukor, Mohd Yunus Shamaan, Nor Aripin Khalid, Ariff Ganzau, Azlan Jualang Sulaiman, Mohd Rosni Jirangon, Hussain Ahmad, Siti Aqlima The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants. Friends Science Publishers 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46049/1/Purification%20and%20anticholinesterase%20sensitivity%20of%20cholinesterase%20extracted%20from%20liver%20tissue%20of%20Puntius%20javanicus.pdf Sabullah, Mohd Khalizan and Abd. Shukor, Mohd Yunus and Shamaan, Nor Aripin and Khalid, Ariff and Ganzau, Azlan Jualang and Sulaiman, Mohd Rosni and Jirangon, Hussain and Ahmad, Siti Aqlima (2015) Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus. International Journal of Agriculture and Biology, 17 (5). pp. 1025-1030. ISSN 1560-8530; ESSN: 1814-9596 http://www.fspublishers.org 10.17957/IJAB/15.0012
institution UPM IR
collection UPM IR
language English
description The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants.
format Article
author Sabullah, Mohd Khalizan
Abd. Shukor, Mohd Yunus
Shamaan, Nor Aripin
Khalid, Ariff
Ganzau, Azlan Jualang
Sulaiman, Mohd Rosni
Jirangon, Hussain
Ahmad, Siti Aqlima
spellingShingle Sabullah, Mohd Khalizan
Abd. Shukor, Mohd Yunus
Shamaan, Nor Aripin
Khalid, Ariff
Ganzau, Azlan Jualang
Sulaiman, Mohd Rosni
Jirangon, Hussain
Ahmad, Siti Aqlima
Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
author_facet Sabullah, Mohd Khalizan
Abd. Shukor, Mohd Yunus
Shamaan, Nor Aripin
Khalid, Ariff
Ganzau, Azlan Jualang
Sulaiman, Mohd Rosni
Jirangon, Hussain
Ahmad, Siti Aqlima
author_sort Sabullah, Mohd Khalizan
title Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
title_short Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
title_full Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
title_fullStr Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
title_full_unstemmed Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
title_sort purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of puntius javanicus
publisher Friends Science Publishers
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/46049/1/Purification%20and%20anticholinesterase%20sensitivity%20of%20cholinesterase%20extracted%20from%20liver%20tissue%20of%20Puntius%20javanicus.pdf
_version_ 1819296692066844672
score 13.4562235

Documenti analoghi