TY - JOUR T1 - Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus A1 - Roowi, Suri LA - English PB - Malaysian Agricultural Research and Development Institute (MARDI) YR - 2002 UL - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:48930 AB - Treatment of Citrus hystrix callus with various types of elicitors decreased the callus growth as the concentration of elicitors increased. However, callus growth remained relatively constant at 0.05% (w/v) alginate and slightly increased to 1.30 ± 0.04 g dwt./culture at 0.1% (w/v) alginate. Analysis of the flavonoids using high performance liquid chromatography (HPLC) showed that only naringin and rutin were produced. Maximum production of naringin (12.13 ± 0.07 mg/g dwt.) and rutin (3.09 ± 0.05 mg/g dwt.) (p ≤ 0.05) was found in callus treated with 0.5% (w/v) agarose. ER -