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Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak

Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing b...

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Main Authors: Sabullah, Mohd Khalizan, Rahman, Mohd Fadhil, Ahmad, Siti Aqlima, Sulaiman, Mohd Rosni, Shukor, Mohd Shukri, Shamaan, Nor Aripin, Abd Shukor, Mohd Yunus
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Teanga:English
Foilsithe: Faculty of Agriculture, University of Brawijaya, Indonesia 2016
Rochtain Ar Líne:http://psasir.upm.edu.my/id/eprint/54629/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20SAW-5%20in%20solid%20from%20Sarawak%20.pdf
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spelling oai:psasir.upm.edu.my:54629 http://psasir.upm.edu.my/id/eprint/54629/ Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak Sabullah, Mohd Khalizan Rahman, Mohd Fadhil Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Abd Shukor, Mohd Yunus Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. Faculty of Agriculture, University of Brawijaya, Indonesia 2016-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54629/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20SAW-5%20in%20solid%20from%20Sarawak%20.pdf Sabullah, Mohd Khalizan and Rahman, Mohd Fadhil and Ahmad, Siti Aqlima and Sulaiman, Mohd Rosni and Shukor, Mohd Shukri and Shamaan, Nor Aripin and Abd Shukor, Mohd Yunus (2016) Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak. Agrivita, 38 (1). pp. 1-13. ISSN 0126-0537; ESSN: 2477-8516 http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/654 10.17503/agrivita.v38i1.654
institution UPM IR
collection UPM IR
language English
description Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.
format Article
author Sabullah, Mohd Khalizan
Rahman, Mohd Fadhil
Ahmad, Siti Aqlima
Sulaiman, Mohd Rosni
Shukor, Mohd Shukri
Shamaan, Nor Aripin
Abd Shukor, Mohd Yunus
spellingShingle Sabullah, Mohd Khalizan
Rahman, Mohd Fadhil
Ahmad, Siti Aqlima
Sulaiman, Mohd Rosni
Shukor, Mohd Shukri
Shamaan, Nor Aripin
Abd Shukor, Mohd Yunus
Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
author_facet Sabullah, Mohd Khalizan
Rahman, Mohd Fadhil
Ahmad, Siti Aqlima
Sulaiman, Mohd Rosni
Shukor, Mohd Shukri
Shamaan, Nor Aripin
Abd Shukor, Mohd Yunus
author_sort Sabullah, Mohd Khalizan
title Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
title_short Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
title_full Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
title_fullStr Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
title_full_unstemmed Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
title_sort isolation and characterisation of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in solid from sarawak
publisher Faculty of Agriculture, University of Brawijaya, Indonesia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54629/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20SAW-5%20in%20solid%20from%20Sarawak%20.pdf
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score 13.4562235

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