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Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos

The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes in morphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fi...

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Principais autores: Karami, Ali, Goh, Yong Meng, Jahromi, Mohammad Faseleh, James Lazorchak, Ali, Abdullah, Maha, Courtenay, Simon C.
Formato: Atigo
Idioma:English
Publicado em: Elsevier 2016
Acesso em linha:http://psasir.upm.edu.my/id/eprint/55532/1/Diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20differ%20in%20biomarker%20responses%20.pdf
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spelling oai:psasir.upm.edu.my:55532 http://psasir.upm.edu.my/id/eprint/55532/ Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos Karami, Ali Goh, Yong Meng Jahromi, Mohammad Faseleh James Lazorchak, Ali Abdullah, Maha Courtenay, Simon C. The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes in morphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fish were exposed to three concentrations of CPF (mean measured μg/L (SD): 9.71 (2.27), 15.7 (3.69), 31.21 (5.04)) under a static-renewal condition for 21 days. Diploid control groups had higher hepatosomatic index (HSI), plasma testosterone (T), and brain GnRH and cyp19a2 expression levels than triploids. In CPF-exposed groups, changes in HSI, total weight and length were different between the diploid and triploid fish. In contrast, condition factor did not alter in any of the treatments, while visceral-somatic index (VSI) changed only in diploids. In diploid fish, exposure to CPF did not change brain 11β-hsd2, ftz-f1, foxl2, GnRH or cyp19a2 mRNA levels, while reduced tph2 transcript levels compared to the control group. In contrast, 11β-hsd2 and foxl2 expression levels were changed in triploids following CPF exposures. In diploids, plasma T levels showed a linear dose-response reduction across CPF treatments correlating with liver weight and plasma total cholesterol concentrations. In contrast, no changes in plasma cholesterol and T concentrations were observed in triploids. Plasma cortisol and 17-β estradiol (E2) showed no response to CPF exposure in either ploidy. Results of this first comparison of biomarker responses to pesticide exposure in diploid and polyploid animals showed substantial differences between diploid and triploid C. gariepinus. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55532/1/Diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20differ%20in%20biomarker%20responses%20.pdf Karami, Ali and Goh, Yong Meng and Jahromi, Mohammad Faseleh and James Lazorchak, Ali and Abdullah, Maha and Courtenay, Simon C. (2016) Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos. Science of The Total Environment, 557558 (July). pp. 204-211. ISSN 0048-9697; ESSN: 1879-1026 10.1016/j.scitotenv.2016.03.030
institution UPM IR
collection UPM IR
language English
description The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes in morphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fish were exposed to three concentrations of CPF (mean measured μg/L (SD): 9.71 (2.27), 15.7 (3.69), 31.21 (5.04)) under a static-renewal condition for 21 days. Diploid control groups had higher hepatosomatic index (HSI), plasma testosterone (T), and brain GnRH and cyp19a2 expression levels than triploids. In CPF-exposed groups, changes in HSI, total weight and length were different between the diploid and triploid fish. In contrast, condition factor did not alter in any of the treatments, while visceral-somatic index (VSI) changed only in diploids. In diploid fish, exposure to CPF did not change brain 11β-hsd2, ftz-f1, foxl2, GnRH or cyp19a2 mRNA levels, while reduced tph2 transcript levels compared to the control group. In contrast, 11β-hsd2 and foxl2 expression levels were changed in triploids following CPF exposures. In diploids, plasma T levels showed a linear dose-response reduction across CPF treatments correlating with liver weight and plasma total cholesterol concentrations. In contrast, no changes in plasma cholesterol and T concentrations were observed in triploids. Plasma cortisol and 17-β estradiol (E2) showed no response to CPF exposure in either ploidy. Results of this first comparison of biomarker responses to pesticide exposure in diploid and polyploid animals showed substantial differences between diploid and triploid C. gariepinus.
format Article
author Karami, Ali
Goh, Yong Meng
Jahromi, Mohammad Faseleh
James Lazorchak, Ali
Abdullah, Maha
Courtenay, Simon C.
spellingShingle Karami, Ali
Goh, Yong Meng
Jahromi, Mohammad Faseleh
James Lazorchak, Ali
Abdullah, Maha
Courtenay, Simon C.
Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
author_facet Karami, Ali
Goh, Yong Meng
Jahromi, Mohammad Faseleh
James Lazorchak, Ali
Abdullah, Maha
Courtenay, Simon C.
author_sort Karami, Ali
title Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
title_short Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
title_full Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
title_fullStr Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
title_full_unstemmed Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
title_sort diploid and triploid african catfish (clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos
publisher Elsevier
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55532/1/Diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20differ%20in%20biomarker%20responses%20.pdf
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score 13.4562235

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