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Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant

Background The development of a potential single culture that can co-produce hydrogen and ethanol is beneficial for industrial application. Strain improvement via molecular approach was proposed on hydrogen and ethanol co-producing bacterium, Escherichia coli SS1. Thus, the effect of additional cop...

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Hlavní autoři: Soo, Chiu Shyan, Yap, Wai Sum, Hon, Wei Min, Ramli, Norhayati, Md Shah, Umi Kalsom, Phang, Lai-Yee
Médium: Článek
Jazyk:English
Vydáno: Pontificia Universidad Católica de Valparaíso 2017
On-line přístup:http://psasir.upm.edu.my/id/eprint/61229/1/Co-production%20of%20hydrogen%20and%20ethanol%20by%20Escherichia%20coli%20SS1%20and%20its%20recombinant.pdf
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spelling oai:psasir.upm.edu.my:61229 http://psasir.upm.edu.my/id/eprint/61229/ Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant Soo, Chiu Shyan Yap, Wai Sum Hon, Wei Min Ramli, Norhayati Md Shah, Umi Kalsom Phang, Lai-Yee Background The development of a potential single culture that can co-produce hydrogen and ethanol is beneficial for industrial application. Strain improvement via molecular approach was proposed on hydrogen and ethanol co-producing bacterium, Escherichia coli SS1. Thus, the effect of additional copy of native hydrogenase gene hybC on hydrogen and ethanol co-production by E. coli SS1 was investigated. Results Both E. coli SS1 and the recombinant hybC were subjected to fermentation using 10 g/L of glycerol at initial pH 7.5. Recombinant hybC had about 2-fold higher cell growth, 5.2-fold higher glycerol consumption rate and 3-fold higher ethanol productivity in comparison to wild-type SS1. Nevertheless, wild-type SS1 reported hydrogen yield of 0.57 mol/mol glycerol and ethanol yield of 0.88 mol/mol glycerol, which were 4- and 1.4-fold higher in comparison to recombinant hybC. Glucose fermentation was also conducted for comparison study. The performance of wild-type SS1 and recombinant hybC showed relatively similar results during glucose fermentation. Additional copy of hybC gene could manipulate the glycerol metabolic pathway of E. coli SS1 under slightly alkaline condition. Conclusions HybC could improve glycerol consumption rate and ethanol productivity of E. coli despite lower hydrogen and ethanol yields. Higher glycerol consumption rate of recombinant hybC could be an advantage for bioconversion of glycerol into biofuels. This study could serve as a useful guidance for dissecting the role of hydrogenase in glycerol metabolism and future development of effective strain for biofuels production. Pontificia Universidad Católica de Valparaíso 2017-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61229/1/Co-production%20of%20hydrogen%20and%20ethanol%20by%20Escherichia%20coli%20SS1%20and%20its%20recombinant.pdf Soo, Chiu Shyan and Yap, Wai Sum and Hon, Wei Min and Ramli, Norhayati and Md Shah, Umi Kalsom and Phang, Lai-Yee (2017) Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant. Electronic Journal of Biotechnology, 30. 64 - 70. ISSN 0717-3458 10.1016/j.ejbt.2017.09.002
institution UPM IR
collection UPM IR
language English
description Background The development of a potential single culture that can co-produce hydrogen and ethanol is beneficial for industrial application. Strain improvement via molecular approach was proposed on hydrogen and ethanol co-producing bacterium, Escherichia coli SS1. Thus, the effect of additional copy of native hydrogenase gene hybC on hydrogen and ethanol co-production by E. coli SS1 was investigated. Results Both E. coli SS1 and the recombinant hybC were subjected to fermentation using 10 g/L of glycerol at initial pH 7.5. Recombinant hybC had about 2-fold higher cell growth, 5.2-fold higher glycerol consumption rate and 3-fold higher ethanol productivity in comparison to wild-type SS1. Nevertheless, wild-type SS1 reported hydrogen yield of 0.57 mol/mol glycerol and ethanol yield of 0.88 mol/mol glycerol, which were 4- and 1.4-fold higher in comparison to recombinant hybC. Glucose fermentation was also conducted for comparison study. The performance of wild-type SS1 and recombinant hybC showed relatively similar results during glucose fermentation. Additional copy of hybC gene could manipulate the glycerol metabolic pathway of E. coli SS1 under slightly alkaline condition. Conclusions HybC could improve glycerol consumption rate and ethanol productivity of E. coli despite lower hydrogen and ethanol yields. Higher glycerol consumption rate of recombinant hybC could be an advantage for bioconversion of glycerol into biofuels. This study could serve as a useful guidance for dissecting the role of hydrogenase in glycerol metabolism and future development of effective strain for biofuels production.
format Article
author Soo, Chiu Shyan
Yap, Wai Sum
Hon, Wei Min
Ramli, Norhayati
Md Shah, Umi Kalsom
Phang, Lai-Yee
spellingShingle Soo, Chiu Shyan
Yap, Wai Sum
Hon, Wei Min
Ramli, Norhayati
Md Shah, Umi Kalsom
Phang, Lai-Yee
Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
author_facet Soo, Chiu Shyan
Yap, Wai Sum
Hon, Wei Min
Ramli, Norhayati
Md Shah, Umi Kalsom
Phang, Lai-Yee
author_sort Soo, Chiu Shyan
title Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
title_short Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
title_full Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
title_fullStr Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
title_full_unstemmed Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
title_sort co-production of hydrogen and ethanol by escherichia coli ss1 and its recombinant
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61229/1/Co-production%20of%20hydrogen%20and%20ethanol%20by%20Escherichia%20coli%20SS1%20and%20its%20recombinant.pdf
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score 13.4562235

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