תמונות העטיפה

TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding

Induced mutations have been used effectively for plant improvement. Physical and chemical mutagens induce a high frequency of genome variation. Recently, developed screening methods have allowed the detection of single nucleotide polymorphisms (SNPs) and the identification of traits that are difficu...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Taheri, Sima, Abdullah, Thohirah Lee, Jain, Shri, Sahebi, Mahbod, Azizi, Parisa
פורמט: Article
שפה:English
יצא לאור: Springer 2017
גישה מקוונת:http://psasir.upm.edu.my/id/eprint/63575/1/TILLING%2C%20high-resolution%20melting%20%28HRM%29%2C%20and%20next-generation%20sequencing%20%28NGS%29%20techniques%20in%20plant%20mutation%20breeding.pdf
תגים: הוספת תג
אין תגיות, היה/י הראשונ/ה לתייג את הרשומה!
id oai:psasir.upm.edu.my:63575
record_format eprints
spelling oai:psasir.upm.edu.my:63575 http://psasir.upm.edu.my/id/eprint/63575/ TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding Taheri, Sima Abdullah, Thohirah Lee Jain, Shri Sahebi, Mahbod Azizi, Parisa Induced mutations have been used effectively for plant improvement. Physical and chemical mutagens induce a high frequency of genome variation. Recently, developed screening methods have allowed the detection of single nucleotide polymorphisms (SNPs) and the identification of traits that are difficult to identify at the molecular level by conventional breeding. With the assistance of reverse genetic techniques, sequence variation information can be linked to traits to investigate gene function. Targeting induced local lesions in genomes (TILLING) is a high-throughput technique to identify single nucleotide mutations in a specific region of a gene of interest with a powerful detection method resulted from chemical-induced mutagenesis. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of genome size and ploidy level. However, TILLING requires laborious and time-consuming steps, and a lack of complete genome sequence information for many crop species has slowed the development of suitable TILLING targets. Another method, high-resolution melting (HRM), which has assisted TILLING in mutation detection, is faster, simpler and less expensive with non-enzymatic screening system. Currently, the sequencing of crop genomes has completely changed our vision and interpretation of genome organization and evolution. Impressive progress in next-generation sequencing (NGS) technologies has paved the way for the detection and exploitation of genetic variation in a given DNA or RNA molecule. This review discusses the applications of TILLING in combination with HRM and NGS technologies for screening of induced mutations and discovering SNPs in mutation breeding programs. Springer 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63575/1/TILLING%2C%20high-resolution%20melting%20%28HRM%29%2C%20and%20next-generation%20sequencing%20%28NGS%29%20techniques%20in%20plant%20mutation%20breeding.pdf Taheri, Sima and Abdullah, Thohirah Lee and Jain, Shri and Sahebi, Mahbod and Azizi, Parisa (2017) TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding. Molecular Breeding, 37 (40). pp. 1-23. ISSN 1380-3743; ESSN: 1572-9788 https://link.springer.com/article/10.1007/s11032-017-0643-7 10.1007/s11032-017-0643-7
institution UPM IR
collection UPM IR
language English
description Induced mutations have been used effectively for plant improvement. Physical and chemical mutagens induce a high frequency of genome variation. Recently, developed screening methods have allowed the detection of single nucleotide polymorphisms (SNPs) and the identification of traits that are difficult to identify at the molecular level by conventional breeding. With the assistance of reverse genetic techniques, sequence variation information can be linked to traits to investigate gene function. Targeting induced local lesions in genomes (TILLING) is a high-throughput technique to identify single nucleotide mutations in a specific region of a gene of interest with a powerful detection method resulted from chemical-induced mutagenesis. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of genome size and ploidy level. However, TILLING requires laborious and time-consuming steps, and a lack of complete genome sequence information for many crop species has slowed the development of suitable TILLING targets. Another method, high-resolution melting (HRM), which has assisted TILLING in mutation detection, is faster, simpler and less expensive with non-enzymatic screening system. Currently, the sequencing of crop genomes has completely changed our vision and interpretation of genome organization and evolution. Impressive progress in next-generation sequencing (NGS) technologies has paved the way for the detection and exploitation of genetic variation in a given DNA or RNA molecule. This review discusses the applications of TILLING in combination with HRM and NGS technologies for screening of induced mutations and discovering SNPs in mutation breeding programs.
format Article
author Taheri, Sima
Abdullah, Thohirah Lee
Jain, Shri
Sahebi, Mahbod
Azizi, Parisa
spellingShingle Taheri, Sima
Abdullah, Thohirah Lee
Jain, Shri
Sahebi, Mahbod
Azizi, Parisa
TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
author_facet Taheri, Sima
Abdullah, Thohirah Lee
Jain, Shri
Sahebi, Mahbod
Azizi, Parisa
author_sort Taheri, Sima
title TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
title_short TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
title_full TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
title_fullStr TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
title_full_unstemmed TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
title_sort tilling, high-resolution melting (hrm), and next-generation sequencing (ngs) techniques in plant mutation breeding
publisher Springer
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/63575/1/TILLING%2C%20high-resolution%20melting%20%28HRM%29%2C%20and%20next-generation%20sequencing%20%28NGS%29%20techniques%20in%20plant%20mutation%20breeding.pdf
_version_ 1819298250935500800
score 13.4562235

פריטים דומים