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Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia

Feline Coronaviruses (FCoVs) are widespread in cat populations throughout the world. Infection with FCoV results in a diverse range of signs from no clinical symptom at all to a highly fatal immune-mediated disease called feline infectious peritonitis (FIP). It has been speculated that mutations in...

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Hoofdauteurs: Sharif, Saeed, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman, Allaudin, Zeenathul Nazariah, Hafidz, Mohd Afzal
Formaat: Conference or Workshop Item
Taal:English
Gepubliceerd in: 2009
Online toegang:http://psasir.upm.edu.my/id/eprint/65138/1/PGM-1-19.pdf
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spelling oai:psasir.upm.edu.my:65138 http://psasir.upm.edu.my/id/eprint/65138/ Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia Sharif, Saeed Arshad, Siti Suri Bejo, Mohd Hair Omar, Abdul Rahman Allaudin, Zeenathul Nazariah Hafidz, Mohd Afzal Feline Coronaviruses (FCoVs) are widespread in cat populations throughout the world. Infection with FCoV results in a diverse range of signs from no clinical symptom at all to a highly fatal immune-mediated disease called feline infectious peritonitis (FIP). It has been speculated that mutations in the FCoV genome induce virulent variants in infected cats and lead to the development of FIP. In present study, a 223 bp region of 3’UTR of four FCoVs isolated from healthy cats was amplified by a one-step reverse transcriptase polymerase chain reaction (RTPCR) and sequenced. The nucleotide sequences were aligned with those of FCoV reference strains and identity and phylogenetic tree were determined. The obtained sequence of Malaysian FCoV isolates revealed 94.6 % identity with a few point mutations. The extent of identity decreased to 86.6 % when compared with the sequence of other FCoV strains/isolates. The overall phylogenetic analysis based on this comparison showed that there are two distinct clusters. The four local isolates are shown to be closely related and fall together in one of the clusters. This study provided the first genetic data on FCoV isolates from Malaysia. 2009 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65138/1/PGM-1-19.pdf Sharif, Saeed and Arshad, Siti Suri and Bejo, Mohd Hair and Omar, Abdul Rahman and Allaudin, Zeenathul Nazariah and Hafidz, Mohd Afzal (2009) Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia. In: 8th Malaysia Congress on Genetics, 4-6 Aug. 2009, Genting Highlands, Malaysia. (pp. 314-318).
institution UPM IR
collection UPM IR
language English
description Feline Coronaviruses (FCoVs) are widespread in cat populations throughout the world. Infection with FCoV results in a diverse range of signs from no clinical symptom at all to a highly fatal immune-mediated disease called feline infectious peritonitis (FIP). It has been speculated that mutations in the FCoV genome induce virulent variants in infected cats and lead to the development of FIP. In present study, a 223 bp region of 3’UTR of four FCoVs isolated from healthy cats was amplified by a one-step reverse transcriptase polymerase chain reaction (RTPCR) and sequenced. The nucleotide sequences were aligned with those of FCoV reference strains and identity and phylogenetic tree were determined. The obtained sequence of Malaysian FCoV isolates revealed 94.6 % identity with a few point mutations. The extent of identity decreased to 86.6 % when compared with the sequence of other FCoV strains/isolates. The overall phylogenetic analysis based on this comparison showed that there are two distinct clusters. The four local isolates are shown to be closely related and fall together in one of the clusters. This study provided the first genetic data on FCoV isolates from Malaysia.
format Conference or Workshop Item
author Sharif, Saeed
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Allaudin, Zeenathul Nazariah
Hafidz, Mohd Afzal
spellingShingle Sharif, Saeed
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Allaudin, Zeenathul Nazariah
Hafidz, Mohd Afzal
Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia
author_facet Sharif, Saeed
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Allaudin, Zeenathul Nazariah
Hafidz, Mohd Afzal
author_sort Sharif, Saeed
title Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia
title_short Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia
title_full Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia
title_fullStr Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia
title_full_unstemmed Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia
title_sort phylogenetic analysis of feline coronavirus isolates from healthy cats in malaysia
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/65138/1/PGM-1-19.pdf
_version_ 1819298378636328960
score 13.4562235

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