TY  - JOUR
T1  - An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production
A1  - Raja Abdul Rahman, Raja Noor Zaliha
LA  - English
PB  - Elsevier
YR  - 2005
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:7058
AB  - An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbitol as the sole carbon source, followed by starch and lactose at pH 7.0 and 37 °C. Dextrose, sucrose and glycerol greatly reduced the protease production. The best organic nitrogen source was casamino acid. Tryptone, soytone and yeast extract supported protease production while corn  steep liquor and beef extract inhibited the protease activity. Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it. More than 62% drop in production occurred in the presence of amino acids. Addition of metal ions such as K+, Mg2+ and Ca2+ maximized the enzyme production.
ER  -