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Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture

Induced pluripotent stem cells (iPSCs) have great potentials for regenerative medicine. However, serious concerns such as the use of the viral-mediated reprogramming strategies and exposure of iPSCs to animal products from feeder cells and serum-containing medium have restricted the application of i...

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Main Authors: Al Abbar, Akram Faisal Mahmaud, Nordin, Norshariza, Ghazalli, Nadiah, Abdullah, Syahrilnizam
格式: Article
语言:English
出版: Elsevier 2018
在线阅读:http://psasir.upm.edu.my/id/eprint/72831/1/Generation%20of%20induced%20pluripotent%20stem%20cells%20.pdf
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spelling oai:psasir.upm.edu.my:72831 http://psasir.upm.edu.my/id/eprint/72831/ Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture Al Abbar, Akram Faisal Mahmaud Nordin, Norshariza Ghazalli, Nadiah Abdullah, Syahrilnizam Induced pluripotent stem cells (iPSCs) have great potentials for regenerative medicine. However, serious concerns such as the use of the viral-mediated reprogramming strategies and exposure of iPSCs to animal products from feeder cells and serum-containing medium have restricted the application of iPSCs in the clinics. Therefore, the generation of iPSCs with minimal viral integrations and in non-animal sourced and serum-free medium is necessary. In this report, a polycistronic lentiviral vector carrying Yamanaka's factors was used to reprogram mouse fibroblasts into iPSCs in feeder- and xeno-free culture environment. The generated iPSCs exhibited morphology and self-renewal properties of embryonic stem cells (ESCs), expression of specific pluripotent markers, and potentials to differentiate into the three-major distinct specialized germ layers in vitro. The iPSCs were also shown to have the potential to differentiate into neural precursor and neurons in culture, with greater than 95% expression of nestin, Pax6 and βIII-tubulin. This body of work describes an alternative method of generating iPSCs by using polycistronic lentiviral vector that may minimize the risks associated with viral vector-mediated reprogramming and animal derived products in the culture media. Elsevier 2018-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72831/1/Generation%20of%20induced%20pluripotent%20stem%20cells%20.pdf Al Abbar, Akram Faisal Mahmaud and Nordin, Norshariza and Ghazalli, Nadiah and Abdullah, Syahrilnizam (2018) Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture. Tissue and Cell, 55. 13 - 24. ISSN 0040-8166; ESSN: 1532-3072 https://pubmed.ncbi.nlm.nih.gov/30503056/ 10.1016/j.tice.2018.09.004
institution UPM IR
collection UPM IR
language English
description Induced pluripotent stem cells (iPSCs) have great potentials for regenerative medicine. However, serious concerns such as the use of the viral-mediated reprogramming strategies and exposure of iPSCs to animal products from feeder cells and serum-containing medium have restricted the application of iPSCs in the clinics. Therefore, the generation of iPSCs with minimal viral integrations and in non-animal sourced and serum-free medium is necessary. In this report, a polycistronic lentiviral vector carrying Yamanaka's factors was used to reprogram mouse fibroblasts into iPSCs in feeder- and xeno-free culture environment. The generated iPSCs exhibited morphology and self-renewal properties of embryonic stem cells (ESCs), expression of specific pluripotent markers, and potentials to differentiate into the three-major distinct specialized germ layers in vitro. The iPSCs were also shown to have the potential to differentiate into neural precursor and neurons in culture, with greater than 95% expression of nestin, Pax6 and βIII-tubulin. This body of work describes an alternative method of generating iPSCs by using polycistronic lentiviral vector that may minimize the risks associated with viral vector-mediated reprogramming and animal derived products in the culture media.
format Article
author Al Abbar, Akram Faisal Mahmaud
Nordin, Norshariza
Ghazalli, Nadiah
Abdullah, Syahrilnizam
spellingShingle Al Abbar, Akram Faisal Mahmaud
Nordin, Norshariza
Ghazalli, Nadiah
Abdullah, Syahrilnizam
Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
author_facet Al Abbar, Akram Faisal Mahmaud
Nordin, Norshariza
Ghazalli, Nadiah
Abdullah, Syahrilnizam
author_sort Al Abbar, Akram Faisal Mahmaud
title Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
title_short Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
title_full Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
title_fullStr Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
title_full_unstemmed Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
title_sort generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72831/1/Generation%20of%20induced%20pluripotent%20stem%20cells%20.pdf
_version_ 1819299073153302528
score 13.4562235

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