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The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1

The use of probiotic as dietary approach to prevent exposure to food contaminant, aflatoxin B1 (AFB1) has greatly increased. Several studies found that AFB1 binding to the bacterial cell wall is strain-specific. Moreover, the interaction between AFB1 and bacterial cell wall is not well-understood, t...

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Main Authors: Liew, Winnie Pui Pui, Zainuddin, Nurul Adilah, Than, Leslie Thian Lung, Sabran, Mohd Redzwan
Format: Article
Language:English
Published: Frontiers Media 2018
Online Access:http://psasir.upm.edu.my/id/eprint/74116/1/The%20binding%20efficiency%20and%20interaction%20of%20Lactobacillus%20casei%20Shirota%20toward%20aflatoxin%20B1.pdf
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spelling oai:psasir.upm.edu.my:74116 http://psasir.upm.edu.my/id/eprint/74116/ The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1 Liew, Winnie Pui Pui Zainuddin, Nurul Adilah Than, Leslie Thian Lung Sabran, Mohd Redzwan The use of probiotic as dietary approach to prevent exposure to food contaminant, aflatoxin B1 (AFB1) has greatly increased. Several studies found that AFB1 binding to the bacterial cell wall is strain-specific. Moreover, the interaction between AFB1 and bacterial cell wall is not well-understood, thus warrants further investigation. This research was conducted to assess the ability of Lactobacillus casei Shirota (Lcs) to bind AFB1 at different concentrations and to determine AFB1 binding efficiency of different Lcs cell components including live cell, heat-treated, and cell wall. In addition, the interaction between AFB1 and Lcs was also evaluated via scanning electron microscopy (SEM) and through an animal study. The binding of AFB1 by all Lcs cell components depends on the concentration of available AFB1. Among all Lcs cell components, the live Lcs cells exhibited the highest binding efficiency (98%) toward AFB1. Besides, the SEM micrographs showed that AFB1 induced structural changes on the bacterial cell surface and morphology including rough and irregular surface along with a curve rod-shaped. In vivo experiment revealed that Lcs is capable to neutralize the toxicity of AFB1 on body weight and intestine through the binding process. The animal’s growth was stunted due to AFB1 exposure, however, such effect was significantly (p < 0.05) alleviated by Lcs. This phenomenon can be explained by a significant (p < 0.05) decreased level of blood serum AFB1 by Lcs (49.6 ± 8.05 ng/mL) compared to AFB1-exposed rats without treatment (88.12 ± 10.65 ng/mL). Taken together, this study highlights the potential use of Lcs as a preventive agent against aflatoxicosis via its strong binding capability. Frontiers Media 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74116/1/The%20binding%20efficiency%20and%20interaction%20of%20Lactobacillus%20casei%20Shirota%20toward%20aflatoxin%20B1.pdf Liew, Winnie Pui Pui and Zainuddin, Nurul Adilah and Than, Leslie Thian Lung and Sabran, Mohd Redzwan (2018) The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1. Frontiers in Microbiology, 9 (1503). pp. 1-12. ISSN ESSN: 1664-302X https://www.ncbi.nlm.nih.gov/pubmed/30042748 10.3389/fmicb.2018.01503
institution UPM IR
collection UPM IR
language English
description The use of probiotic as dietary approach to prevent exposure to food contaminant, aflatoxin B1 (AFB1) has greatly increased. Several studies found that AFB1 binding to the bacterial cell wall is strain-specific. Moreover, the interaction between AFB1 and bacterial cell wall is not well-understood, thus warrants further investigation. This research was conducted to assess the ability of Lactobacillus casei Shirota (Lcs) to bind AFB1 at different concentrations and to determine AFB1 binding efficiency of different Lcs cell components including live cell, heat-treated, and cell wall. In addition, the interaction between AFB1 and Lcs was also evaluated via scanning electron microscopy (SEM) and through an animal study. The binding of AFB1 by all Lcs cell components depends on the concentration of available AFB1. Among all Lcs cell components, the live Lcs cells exhibited the highest binding efficiency (98%) toward AFB1. Besides, the SEM micrographs showed that AFB1 induced structural changes on the bacterial cell surface and morphology including rough and irregular surface along with a curve rod-shaped. In vivo experiment revealed that Lcs is capable to neutralize the toxicity of AFB1 on body weight and intestine through the binding process. The animal’s growth was stunted due to AFB1 exposure, however, such effect was significantly (p < 0.05) alleviated by Lcs. This phenomenon can be explained by a significant (p < 0.05) decreased level of blood serum AFB1 by Lcs (49.6 ± 8.05 ng/mL) compared to AFB1-exposed rats without treatment (88.12 ± 10.65 ng/mL). Taken together, this study highlights the potential use of Lcs as a preventive agent against aflatoxicosis via its strong binding capability.
format Article
author Liew, Winnie Pui Pui
Zainuddin, Nurul Adilah
Than, Leslie Thian Lung
Sabran, Mohd Redzwan
spellingShingle Liew, Winnie Pui Pui
Zainuddin, Nurul Adilah
Than, Leslie Thian Lung
Sabran, Mohd Redzwan
The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1
author_facet Liew, Winnie Pui Pui
Zainuddin, Nurul Adilah
Than, Leslie Thian Lung
Sabran, Mohd Redzwan
author_sort Liew, Winnie Pui Pui
title The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1
title_short The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1
title_full The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1
title_fullStr The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1
title_full_unstemmed The binding efficiency and interaction of Lactobacillus casei Shirota toward aflatoxin B1
title_sort binding efficiency and interaction of lactobacillus casei shirota toward aflatoxin b1
publisher Frontiers Media
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/74116/1/The%20binding%20efficiency%20and%20interaction%20of%20Lactobacillus%20casei%20Shirota%20toward%20aflatoxin%20B1.pdf
_version_ 1819299150120878080
score 13.4562235

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