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Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)

This study was conducted to evaluate the cross-amplification of six Y chromosome-specific markers that were isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus). All the six markers failed to yield positive results in Malayan tapirs with no amplification (Eca.YM2), and with amplif...

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Autors principals: Tan, Y. L., Annavi, Geetha
Format: Conference or Workshop Item
Idioma:English
Publicat: Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016
Accés en línia:http://psasir.upm.edu.my/id/eprint/77576/1/i-SIMBIOMAS%202016%2084.pdf
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id oai:psasir.upm.edu.my:77576
record_format eprints
spelling oai:psasir.upm.edu.my:77576 http://psasir.upm.edu.my/id/eprint/77576/ Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus) Tan, Y. L. Annavi, Geetha This study was conducted to evaluate the cross-amplification of six Y chromosome-specific markers that were isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus). All the six markers failed to yield positive results in Malayan tapirs with no amplification (Eca.YM2), and with amplification of single band (Eca.YP9) or multiple bands (Eca.YJ10, Eca.YH12, Eca.YE1 and Eca.YA16) in both male and female individuals. Twelve faecal samples were collected from five male individuals of Malayan tapir and stored in three different storage methods (frozen, DET and 80% ethanol). DNA was extracted using a modified protocol of Zhang et al. (2015), yielded DNA concentrations that were not statistically different among the three storage methods based on one-way ANOVA test. Scoring of molecular markers was not carried out because none of the six markers gave a positive result and therefore this step was discontinued. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77576/1/i-SIMBIOMAS%202016%2084.pdf Tan, Y. L. and Annavi, Geetha (2016) Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus). In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 84).
institution UPM IR
collection UPM IR
language English
description This study was conducted to evaluate the cross-amplification of six Y chromosome-specific markers that were isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus). All the six markers failed to yield positive results in Malayan tapirs with no amplification (Eca.YM2), and with amplification of single band (Eca.YP9) or multiple bands (Eca.YJ10, Eca.YH12, Eca.YE1 and Eca.YA16) in both male and female individuals. Twelve faecal samples were collected from five male individuals of Malayan tapir and stored in three different storage methods (frozen, DET and 80% ethanol). DNA was extracted using a modified protocol of Zhang et al. (2015), yielded DNA concentrations that were not statistically different among the three storage methods based on one-way ANOVA test. Scoring of molecular markers was not carried out because none of the six markers gave a positive result and therefore this step was discontinued.
format Conference or Workshop Item
author Tan, Y. L.
Annavi, Geetha
spellingShingle Tan, Y. L.
Annavi, Geetha
Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)
author_facet Tan, Y. L.
Annavi, Geetha
author_sort Tan, Y. L.
title Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)
title_short Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)
title_full Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)
title_fullStr Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)
title_full_unstemmed Cross-amplification of Y chromosome-specific markers isolated from horse (Equus caballus) in Malayan tapir (Tapirus indicus)
title_sort cross-amplification of y chromosome-specific markers isolated from horse (equus caballus) in malayan tapir (tapirus indicus)
publisher Department of Biology, Faculty of Science, Universiti Putra Malaysia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/77576/1/i-SIMBIOMAS%202016%2084.pdf
_version_ 1819299447006298112
score 13.4562235

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