The clinicopathological evaluation of 24 hours upon challenge of Streptococcus iniae in red hybrid tilapia (Oreochromis sp.)
This study was aimed to describe the severity and immunolocalisation of the antigen of lesions in the brain, eyes and kidney in every 6 hours for 24 hours by presence or absence of stress factors before infection. Fifteen Red hybrid tilapia in duplicates were inoculated intraperitoneally with...
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| 主要作者: | |
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| 格式: | Project Paper Report |
| 语言: | English |
| 出版: |
2016
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/78221/1/FPV%202016%2084%20-%20IR.pdf |
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| 总结: | This study was aimed to describe the severity and immunolocalisation of the
antigen of lesions in the brain, eyes and kidney in every 6 hours for 24 hours by
presence or absence of stress factors before infection. Fifteen Red hybrid tilapia in
duplicates were inoculated intraperitoneally with 109 CFU/mL diluted in PBS
while another set, was kept for negative control. Clinical signs were recorded and
observed, and samples from gills, brain, eyes and kidney were collected. Each of
the samples was subjected to bacterial culture and isolation and histopathology.
Immunohistochemistry (IHC) and polymerase chain reaction (PCR) were also
done to detect the presence of the antigen. However, there were no obvious signs, and no macroscopic finding can be observed throughout 24 hours post challenge
(hpc) of the disease. IHC were detected as early as PCR and isolation with
intense staining in a blood vessel, lumen and wall, macrophages in the choroid,
focal haemorrhages in the renal interstitium and meninges especially in heat
stressed followed by no stressors. The immunolocalisation of the antigen is
explained in the pathogenesis of streptococcosis in red tilapia. In conclusion, fish
that is stressed are more likely to develop diseases and showing more severe signs
of disease compared to fish that was not subjected to stress. |
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