TY  - GEN
T1  - Determination of post-mortem interval (PMI) via immunohistochemical localization and expression of biogenic amine (cadaverine)
A1  - Yeow, Mei Juan
LA  - English
YR  - 2017
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:78317
AB  - A dearth of knowledge still exists despite extensive research being conducted 
on a reliable post mortem interval (PMI) indicator. Biogenic amine has never been 
attempted as an indicator of PMI and is evaluated in this study based on 
immunologically expressed cadaverine. Samples of the brain, liver, muscle and testis 
obtained from three dogs at 0, 12th, 18th, and 24th hour post-euthanasia were 
immediately fixed in 10% formalin and routinely processed for histology 
(Haematoxylin and Eosin) and cadaverine immunohistochemistry (IHC). The H&E
stained samples were also subjected to morphometric analysis. Comparable 
cadaverine expressions found at 18th and 24th hour post-mortem were higher than 0 
and 12th hour (p<0.01). Hepatic cadaverine expression surpassed other tissues, except of the brain (p<0.01). However, a time-dependent effect was found on all organs tested 
(p<0.01). The morphometry of hepatocytes, neurons and seminiferous tubules were 
significantly different (p<0.01). In conclusion, it appears that cadaverine expression 
may serve as a PMI indicator. However, similar studies should be subjected to 
different biogenic amines along with its exact quantification.
ER  -