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Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice

Background and Aim: Laboratory mice are widely used as a research model to provide insights into toxicological studies of various xenobiotic. Acetaminophen (APAP) is an antipyretic and analgesic drug that is commonly known as paracetamol, an ideal hepatotoxicant to exhibit centrilobular necrosis in...

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প্রধান লেখক: Fazil, Muhammad Azam, Saulol Hamid, Nur Fazila, Raslam, Nurul Ain Fatin, Mohamed Mustapha, Noordin, Degu, Nurhusien Yimer
বিন্যাস: প্রবন্ধ
ভাষা:English
প্রকাশিত: Veterinary World 2019
অনলাইন ব্যবহার করুন:http://psasir.upm.edu.my/id/eprint/80069/1/Histopathological%20changes%20of%20acetaminophen-induced%20liver%20injury%20and%20subsequent%20liver%20regeneration%20in%20BALBC%20and%20ICR%20mice.pdf
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spelling oai:psasir.upm.edu.my:80069 http://psasir.upm.edu.my/id/eprint/80069/ Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice Fazil, Muhammad Azam Saulol Hamid, Nur Fazila Raslam, Nurul Ain Fatin Mohamed Mustapha, Noordin Degu, Nurhusien Yimer Background and Aim: Laboratory mice are widely used as a research model to provide insights into toxicological studies of various xenobiotic. Acetaminophen (APAP) is an antipyretic and analgesic drug that is commonly known as paracetamol, an ideal hepatotoxicant to exhibit centrilobular necrosis in laboratory mice to resemble humans. However, assessment of histopathological changes between mouse strains is important to decide the optimal mouse model used in APAP toxicity study. Therefore, we aim to assess the histomorphological features of APAP-induced liver injury (AILI) in BALB/C and Institute of Cancer Research (ICR) mice. Materials and Methods: Twenty-five ICR mice and 20 BALB/C mice were used with five animals as controls and the rest were randomly divided into three time points at 5, 24, and 48 hours post-dosing (hpd). They were induced with 500 mg/kg APAP intraperitoneally. Liver sections were processed for hematoxylin-eosin staining and histopathological changes were scored based on grading methods. Results: Intense centrilobular damage was observed as early as 5 hpd in BALB/C as compared to ICR mice, which was observed at 10 hpd. The difference of liver injury between ICR and BALB/C mice is due to dissimilarity in the genetic line-up that related to different elimination pathways of APAP toxicity. However, at 24 hpd, the damage was markedly subsided and liver regeneration had taken place for both ICR and BALB/C groups with evidence of mitotic figures. This study showed that normal liver architecture was restored after the clearance of toxic insult. Conclusion: AILI was exhibited earlier in BALB/C than ICR mice but both underwent liver recovery at later time points. Veterinary World 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80069/1/Histopathological%20changes%20of%20acetaminophen-induced%20liver%20injury%20and%20subsequent%20liver%20regeneration%20in%20BALBC%20and%20ICR%20mice.pdf Fazil, Muhammad Azam and Saulol Hamid, Nur Fazila and Raslam, Nurul Ain Fatin and Mohamed Mustapha, Noordin and Degu, Nurhusien Yimer (2019) Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice. Veterinary World, 12 (11). pp. 1682-1688. ISSN 0972-8988; ESSN: 2231-0916 https://www.researchgate.net/publication/337007289_Histopathological_changes_of_acetaminophen-induced_liver_injury_and_subsequent_liver_regeneration_in_BALBC_and_ICR_mice 10.14202/vetworld.2019.1682-1688
institution UPM IR
collection UPM IR
language English
description Background and Aim: Laboratory mice are widely used as a research model to provide insights into toxicological studies of various xenobiotic. Acetaminophen (APAP) is an antipyretic and analgesic drug that is commonly known as paracetamol, an ideal hepatotoxicant to exhibit centrilobular necrosis in laboratory mice to resemble humans. However, assessment of histopathological changes between mouse strains is important to decide the optimal mouse model used in APAP toxicity study. Therefore, we aim to assess the histomorphological features of APAP-induced liver injury (AILI) in BALB/C and Institute of Cancer Research (ICR) mice. Materials and Methods: Twenty-five ICR mice and 20 BALB/C mice were used with five animals as controls and the rest were randomly divided into three time points at 5, 24, and 48 hours post-dosing (hpd). They were induced with 500 mg/kg APAP intraperitoneally. Liver sections were processed for hematoxylin-eosin staining and histopathological changes were scored based on grading methods. Results: Intense centrilobular damage was observed as early as 5 hpd in BALB/C as compared to ICR mice, which was observed at 10 hpd. The difference of liver injury between ICR and BALB/C mice is due to dissimilarity in the genetic line-up that related to different elimination pathways of APAP toxicity. However, at 24 hpd, the damage was markedly subsided and liver regeneration had taken place for both ICR and BALB/C groups with evidence of mitotic figures. This study showed that normal liver architecture was restored after the clearance of toxic insult. Conclusion: AILI was exhibited earlier in BALB/C than ICR mice but both underwent liver recovery at later time points.
format Article
author Fazil, Muhammad Azam
Saulol Hamid, Nur Fazila
Raslam, Nurul Ain Fatin
Mohamed Mustapha, Noordin
Degu, Nurhusien Yimer
spellingShingle Fazil, Muhammad Azam
Saulol Hamid, Nur Fazila
Raslam, Nurul Ain Fatin
Mohamed Mustapha, Noordin
Degu, Nurhusien Yimer
Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice
author_facet Fazil, Muhammad Azam
Saulol Hamid, Nur Fazila
Raslam, Nurul Ain Fatin
Mohamed Mustapha, Noordin
Degu, Nurhusien Yimer
author_sort Fazil, Muhammad Azam
title Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice
title_short Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice
title_full Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice
title_fullStr Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice
title_full_unstemmed Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice
title_sort histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in balb/c and icr mice
publisher Veterinary World
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80069/1/Histopathological%20changes%20of%20acetaminophen-induced%20liver%20injury%20and%20subsequent%20liver%20regeneration%20in%20BALBC%20and%20ICR%20mice.pdf
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score 13.4562235

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