Knjižničarski pogled: In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells

In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells

Very recently, we postulated that the incorporation of citral into nanostructured lipid carrier (NLC-Citral) improves solubility and delivery of the citral without toxic effects in vivo. Thus, the objective of this study is to evaluate anti-cancer effects of NLC-Citral in MDA MB-231 cells in vitro t...

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Main Authors:	Nordin, Noraini, Yeap, Swee Keong, Muhammad, Heshu Sulaiman Rahman, Zamberi, Nur Rizi, Abu, Nadiah, Mohamad, Nurul Elyani, Chee, Wun How, Masarudin, Mas Jaffri, Abdullah, Rasedee @ Mat, Mohammed Alitheen, Noorjahan Banu
Format:	Article
Jezik:	English
Izdano:	Nature Publishing Group 2019
Online dostop:	http://psasir.upm.edu.my/id/eprint/80222/1/In%20vitro%20cytotoxicity%20and%20anticancer%20effects%20of%20citral%20nanostructured%20lipid%20carrier%20on%20MDA%20MBA-231%20human%20breast%20cancer%20cells.pdf
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spelling	oai:psasir.upm.edu.my:80222 http://psasir.upm.edu.my/id/eprint/80222/ In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells Nordin, Noraini Yeap, Swee Keong Muhammad, Heshu Sulaiman Rahman Zamberi, Nur Rizi Abu, Nadiah Mohamad, Nurul Elyani Chee, Wun How Masarudin, Mas Jaffri Abdullah, Rasedee @ Mat Mohammed Alitheen, Noorjahan Banu Very recently, we postulated that the incorporation of citral into nanostructured lipid carrier (NLC-Citral) improves solubility and delivery of the citral without toxic effects in vivo. Thus, the objective of this study is to evaluate anti-cancer effects of NLC-Citral in MDA MB-231 cells in vitro through the Annexin V, cell cycle, JC-1 and fluorometric assays. Additionally, this study is aimed to effects of NLC-Citral in reducing the tumor weight and size in 4T1 induced murine breast cancer model. Results showed that NLC-Citral induced apoptosis and G2/M arrest in MDA MB-231 cells. Furthermore, a prominent anti-metastatic ability of NLC-Citral was demonstrated in vitro using scratch, migration and invasion assays. A significant reduction of migrated and invaded cells was observed in the NLC-Citral treated MDA MB-231 cells. To further evaluate the apoptotic and anti-metastatic mechanism of NLC-Citral at the molecular level, microarray-based gene expression and proteomic profiling were conducted. Based on the result obtained, NLC-Citral was found to regulate several important signaling pathways related to cancer development such as apoptosis, cell cycle, and metastasis signaling pathways. Additionally, gene expression analysis was validated through the targeted RNA sequencing and real-time polymerase chain reaction. In conclusion, the NLC-Citral inhibited the proliferation of breast cancer cells in vitro, majorly through the induction of apoptosis, anti-metastasis, anti-angiogenesis potentials, and reducing the tumor weight and size without altering the therapeutic effects of citral. Nature Publishing Group 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80222/1/In%20vitro%20cytotoxicity%20and%20anticancer%20effects%20of%20citral%20nanostructured%20lipid%20carrier%20on%20MDA%20MBA-231%20human%20breast%20cancer%20cells.pdf Nordin, Noraini and Yeap, Swee Keong and Muhammad, Heshu Sulaiman Rahman and Zamberi, Nur Rizi and Abu, Nadiah and Mohamad, Nurul Elyani and Chee, Wun How and Masarudin, Mas Jaffri and Abdullah, Rasedee @ Mat and Mohammed Alitheen, Noorjahan Banu (2019) In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells. Scientific Reports, 9. art. no. 1614. pp. 1-19. ISSN 2045-2322 10.1038/s41598-018-38214-x
institution	UPM IR
collection	UPM IR
language	English
description	Very recently, we postulated that the incorporation of citral into nanostructured lipid carrier (NLC-Citral) improves solubility and delivery of the citral without toxic effects in vivo. Thus, the objective of this study is to evaluate anti-cancer effects of NLC-Citral in MDA MB-231 cells in vitro through the Annexin V, cell cycle, JC-1 and fluorometric assays. Additionally, this study is aimed to effects of NLC-Citral in reducing the tumor weight and size in 4T1 induced murine breast cancer model. Results showed that NLC-Citral induced apoptosis and G2/M arrest in MDA MB-231 cells. Furthermore, a prominent anti-metastatic ability of NLC-Citral was demonstrated in vitro using scratch, migration and invasion assays. A significant reduction of migrated and invaded cells was observed in the NLC-Citral treated MDA MB-231 cells. To further evaluate the apoptotic and anti-metastatic mechanism of NLC-Citral at the molecular level, microarray-based gene expression and proteomic profiling were conducted. Based on the result obtained, NLC-Citral was found to regulate several important signaling pathways related to cancer development such as apoptosis, cell cycle, and metastasis signaling pathways. Additionally, gene expression analysis was validated through the targeted RNA sequencing and real-time polymerase chain reaction. In conclusion, the NLC-Citral inhibited the proliferation of breast cancer cells in vitro, majorly through the induction of apoptosis, anti-metastasis, anti-angiogenesis potentials, and reducing the tumor weight and size without altering the therapeutic effects of citral.
format	Article
author	Nordin, Noraini Yeap, Swee Keong Muhammad, Heshu Sulaiman Rahman Zamberi, Nur Rizi Abu, Nadiah Mohamad, Nurul Elyani Chee, Wun How Masarudin, Mas Jaffri Abdullah, Rasedee @ Mat Mohammed Alitheen, Noorjahan Banu
spellingShingle	Nordin, Noraini Yeap, Swee Keong Muhammad, Heshu Sulaiman Rahman Zamberi, Nur Rizi Abu, Nadiah Mohamad, Nurul Elyani Chee, Wun How Masarudin, Mas Jaffri Abdullah, Rasedee @ Mat Mohammed Alitheen, Noorjahan Banu In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
author_facet	Nordin, Noraini Yeap, Swee Keong Muhammad, Heshu Sulaiman Rahman Zamberi, Nur Rizi Abu, Nadiah Mohamad, Nurul Elyani Chee, Wun How Masarudin, Mas Jaffri Abdullah, Rasedee @ Mat Mohammed Alitheen, Noorjahan Banu
author_sort	Nordin, Noraini
title	In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
title_short	In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
title_full	In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
title_fullStr	In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
title_full_unstemmed	In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
title_sort	in vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on mda mba-231 human breast cancer cells
publisher	Nature Publishing Group
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/80222/1/In%20vitro%20cytotoxicity%20and%20anticancer%20effects%20of%20citral%20nanostructured%20lipid%20carrier%20on%20MDA%20MBA-231%20human%20breast%20cancer%20cells.pdf
_version_	1819299657015099392
score	13.4562235

In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells

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