TY  - JOUR
T1  - Simultaneous saccharification and fermentation of sago hampas into biobutanol by Clostridium acetobutylicum ATCC 824
A1  - Husin, Hazwani
LA  - English
PB  - John Wiley & Sons
YR  - 2018
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:psasir.upm.edu.my:82171
AB  - Simultaneous  saccharification  and  fermentation  (SSF)  by  Clostridium  acetobutylicum ATCC 824 was conducted to produce biobutanol from sago hampas. Sago hampas is a waste generated from the processing of sago starch. This waste is composed of 54.6% starch and 31.7% of cellulose and hemicellulose, with only 3.3% of lignin. In order to fully utilize the starch and cellulosic materials, saccharification using a mixture  of  amylase  (Dextrozyme)  and  cellulase  (Acremonium  cellulase)  was  conducted using 0.09 g/mL sago hampas, producing 67.0 g/L of fermentable sugar. The SSF and delayed SSF (DSSF) were conducted using 0.07 g/mL sago hampas with the optimized enzyme loading of Dextrozyme amylase (71.4 U/gsubstrate) and Acremonium cellulase (20 FPU/gsubstrate). The SSF of sago hampas generated 6.12 g/L of solvents with biobutanol concentration of 3.81 g/L and the yield of 0.11 g-    biobutanol/g- sugar. In  order  to  improve  biobutanol  concentration  and  productivity,  DSSF  was  intro-duced. In DSSF, the inoculum was introduced into the system after 24 hour of fermentation  to  allow  the  optimal  saccharification  process  for  sugar  production.  This  process  generated  4.62  g/L  of  biobutanol  which  was  18%  higher  than  normal  SSF  since   the   saccharification   and   fermentation   were   operated   at   their   optimal   condition.
ER  -