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Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line

Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a...

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Egile Nagusiak: Ibiyeye, Kehinde Muibat, Nordin, Norshariza, Ajat, Mokrish, Zakaria Zuki, Abu Bakar
Formatua: Artikulua
Hizkuntza:English
Argitaratua: Frontiers Media 2019
Sarrera elektronikoa:http://psasir.upm.edu.my/id/eprint/82661/1/Ultrastructural%20changes%20and%20antitumor%20.pdf
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spelling oai:psasir.upm.edu.my:82661 http://psasir.upm.edu.my/id/eprint/82661/ Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line Ibiyeye, Kehinde Muibat Nordin, Norshariza Ajat, Mokrish Zakaria Zuki, Abu Bakar Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a combined doxorubicin/thymoquinone-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) on breast cancer cell line and compared with their free drugs counterpart. Methods: Cell viability using MTT assay, apoptosis with Annexin V-PI kit, morphological changes using contrast light microscope, scanning electron microscope and transmission electron microscope, cell cycle analysis, invasion assay, and scratch assay were carried out. The cell viability was evaluated in breast cancer cell line (MDA MB231), normal breast cells (MDF10A) and normal fibroblast (3T3). Results: MDA MB231 IC50 dosages of drug-loaded nanoparticle were not toxic to the normal cells. The combination therapy showed enhanced apoptosis, reduction in cellular migration and invasion when compared to the single drug-loaded nanoparticle and the free drugs. Scanning electron microscope showed presence of cell shrinkage, cell membrane blebbing, while transmission electron microscope showed nuclear fragmentation, disruption of cell membrane, apoptotic bodies, and disruption of mitochondrial cistern. Conclusion: The results from this study showed that the combined drug-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) showed higher efficacy in breast cancer cells at lower dose of doxorubicin and thymoquinone. Frontiers Media 2019-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82661/1/Ultrastructural%20changes%20and%20antitumor%20.pdf Ibiyeye, Kehinde Muibat and Nordin, Norshariza and Ajat, Mokrish and Zakaria Zuki, Abu Bakar (2019) Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line. art. no. 599. pp. 1-14. ISSN 2234-943X https://pubmed.ncbi.nlm.nih.gov/31334120/ 10.3389/fonc.2019.00599
institution UPM IR
collection UPM IR
language English
description Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a combined doxorubicin/thymoquinone-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) on breast cancer cell line and compared with their free drugs counterpart. Methods: Cell viability using MTT assay, apoptosis with Annexin V-PI kit, morphological changes using contrast light microscope, scanning electron microscope and transmission electron microscope, cell cycle analysis, invasion assay, and scratch assay were carried out. The cell viability was evaluated in breast cancer cell line (MDA MB231), normal breast cells (MDF10A) and normal fibroblast (3T3). Results: MDA MB231 IC50 dosages of drug-loaded nanoparticle were not toxic to the normal cells. The combination therapy showed enhanced apoptosis, reduction in cellular migration and invasion when compared to the single drug-loaded nanoparticle and the free drugs. Scanning electron microscope showed presence of cell shrinkage, cell membrane blebbing, while transmission electron microscope showed nuclear fragmentation, disruption of cell membrane, apoptotic bodies, and disruption of mitochondrial cistern. Conclusion: The results from this study showed that the combined drug-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) showed higher efficacy in breast cancer cells at lower dose of doxorubicin and thymoquinone.
format Article
author Ibiyeye, Kehinde Muibat
Nordin, Norshariza
Ajat, Mokrish
Zakaria Zuki, Abu Bakar
spellingShingle Ibiyeye, Kehinde Muibat
Nordin, Norshariza
Ajat, Mokrish
Zakaria Zuki, Abu Bakar
Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
author_facet Ibiyeye, Kehinde Muibat
Nordin, Norshariza
Ajat, Mokrish
Zakaria Zuki, Abu Bakar
author_sort Ibiyeye, Kehinde Muibat
title Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
title_short Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
title_full Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
title_fullStr Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
title_full_unstemmed Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
title_sort ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded caco3 nanoparticles on breast cancer cell line
publisher Frontiers Media
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82661/1/Ultrastructural%20changes%20and%20antitumor%20.pdf
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score 13.4562235

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