Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin

Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a. Initially, the endolysin was cloned using BamHI/XhoI, resulti...

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Main Authors: Tham, Hong Y., Song, Adelene Ai Lian, Yusoff, Khatijah, Tan, Geok Hun
Format: Article
Jezik:English
Izdano: Future Science 2020
Online dostop:http://psasir.upm.edu.my/id/eprint/88588/1/ABSTRACT.pdf
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