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Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats

Citrus hystrix leaf is an important South-East Asian culinary ingredient with anti- oxidant, anti-inflammation, and cardio-protective properties. Inflammation, hyperglycaemia and oxidation are significant contributors to diabetic cataract formation. This study demonstrated the mitigating effects of...

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1. Verfasser: Umran, Nor Shahira Solehah
Format: Abschlussarbeit
Sprache:English
Veröffentlicht: 2019
Schlagworte:
Wound healing - Animal models - Research
Diabetics
Cataract
Online Zugang:http://psasir.upm.edu.my/id/eprint/90408/1/IB%202020%2031%20IR.pdf
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spelling oai:psasir.upm.edu.my:90408 http://psasir.upm.edu.my/id/eprint/90408/ Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats Umran, Nor Shahira Solehah Citrus hystrix leaf is an important South-East Asian culinary ingredient with anti- oxidant, anti-inflammation, and cardio-protective properties. Inflammation, hyperglycaemia and oxidation are significant contributors to diabetic cataract formation. This study demonstrated the mitigating effects of Citrus hystrix leaf extract on diabetes and cataract development in female Sprague Dawley rats. Diabetes was induced by intraperitoneal streptozotocin (75 mg/kg) injection before the rats were orally administrated with the 150 and 300 mg of extract per kg body weight or metformin (250 mg/kg) for 8 weeks after diabetes development. The extract gradually a n d s i g n i f i c a n t l y decreased fasting blood glucose levels (p<0.05), reduced serum malondialdehyde (MDA), prostaglandin E2 (PGE2), vascular endothelial growth factor (VEGF), and tumor necrosis factor alpha (TNF-α) levels. Histological evidence showed the cataracts development were significantly suppressed by the 150 mg/kg extract (p<0.05), performing better than metformin, by ameliorating systemic inflammation (TNF-α, PGE2, VEGF), oxidative stress (malondialdehyde), hyperglycemia and lens- opacification. Good correlations were found between cataract incidence with fasting blood glucose (r²=0.90), serum PGE2 (r²=0.91), MDA (r²=0.99), VEGF (r²=0.71), and TNF-α levels (r²=0.49) suggesting these biomarkers may probably help predict cataract risk. The citrus compounds suppressed PGE2, VEGF, oxidation (MDA) and inflammation to probably prevent fluid influx, lens-fibers osmotic over-hydration, to mitigate diabetic cataract development. This study showed the limonoids and flavonoid rich Citrus hystrix leaf consumption, is a good anti-hyperglycemic/ anti-diabetic agent, with potent anti-oxidant and anti-inflammation properties that help preventing diabetic cataract development. 2019-07 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/90408/1/IB%202020%2031%20IR.pdf Umran, Nor Shahira Solehah (2019) Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats. Masters thesis, Universiti Putra Malaysia. Wound healing - Animal models - Research Diabetics Cataract
institution UPM IR
collection UPM IR
language English
topic Wound healing - Animal models - Research
Diabetics
Cataract
spellingShingle Wound healing - Animal models - Research
Diabetics
Cataract
Umran, Nor Shahira Solehah
Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
description Citrus hystrix leaf is an important South-East Asian culinary ingredient with anti- oxidant, anti-inflammation, and cardio-protective properties. Inflammation, hyperglycaemia and oxidation are significant contributors to diabetic cataract formation. This study demonstrated the mitigating effects of Citrus hystrix leaf extract on diabetes and cataract development in female Sprague Dawley rats. Diabetes was induced by intraperitoneal streptozotocin (75 mg/kg) injection before the rats were orally administrated with the 150 and 300 mg of extract per kg body weight or metformin (250 mg/kg) for 8 weeks after diabetes development. The extract gradually a n d s i g n i f i c a n t l y decreased fasting blood glucose levels (p<0.05), reduced serum malondialdehyde (MDA), prostaglandin E2 (PGE2), vascular endothelial growth factor (VEGF), and tumor necrosis factor alpha (TNF-α) levels. Histological evidence showed the cataracts development were significantly suppressed by the 150 mg/kg extract (p<0.05), performing better than metformin, by ameliorating systemic inflammation (TNF-α, PGE2, VEGF), oxidative stress (malondialdehyde), hyperglycemia and lens- opacification. Good correlations were found between cataract incidence with fasting blood glucose (r²=0.90), serum PGE2 (r²=0.91), MDA (r²=0.99), VEGF (r²=0.71), and TNF-α levels (r²=0.49) suggesting these biomarkers may probably help predict cataract risk. The citrus compounds suppressed PGE2, VEGF, oxidation (MDA) and inflammation to probably prevent fluid influx, lens-fibers osmotic over-hydration, to mitigate diabetic cataract development. This study showed the limonoids and flavonoid rich Citrus hystrix leaf consumption, is a good anti-hyperglycemic/ anti-diabetic agent, with potent anti-oxidant and anti-inflammation properties that help preventing diabetic cataract development.
format Thesis
author Umran, Nor Shahira Solehah
author_facet Umran, Nor Shahira Solehah
author_sort Umran, Nor Shahira Solehah
title Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
title_short Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
title_full Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
title_fullStr Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
title_full_unstemmed Anti-cataract and anti-diabetic properties of Citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
title_sort anti-cataract and anti-diabetic properties of citrus hystrix dc. leaf extract in streptozotocin-induced diabetic rats
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/90408/1/IB%202020%2031%20IR.pdf
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score 13.4562235

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