Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm
The oil palm tissue contains vitamin E in the form of tocotrienols and tocopherols. The combination of prenyldiphosphate and homogentisic acid (HGA) is the first committed step in vitamin E production. The reaction is catalyzed by homogentisate phytyltransferase (HPT). The oil palm genomic sequen...
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| Fformat: | Project Paper Report |
| Iaith: | English |
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2016
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| Mynediad Ar-lein: | http://psasir.upm.edu.my/id/eprint/91427/1/FP%202016%20124%20IR.pdf |
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oai:psasir.upm.edu.my:91427 http://psasir.upm.edu.my/id/eprint/91427/ Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm Yamba, Mohd Saiful The oil palm tissue contains vitamin E in the form of tocotrienols and tocopherols. The combination of prenyldiphosphate and homogentisic acid (HGA) is the first committed step in vitamin E production. The reaction is catalyzed by homogentisate phytyltransferase (HPT). The oil palm genomic sequence containing the HPT gene is used to study the expression of HPT gene in oil palm tissues. The objective of this study is to isolate and clone the HPT promoter sequence. To obtain the result from this study, some molecular work was done, including DNA extraction by DNA extraction kit and the cetyltrimethylammonium bromide (CTAB) method, designing the primer for HPT promoter isolation, running the polymerase chain reaction (PCR) to get the HPT promoter sequence and performing agarose gel purification to purify the HPT promoter sequences. After obtaining the amplified HPT promoter sequence, the HPT promoter was introduced into an entry vector and then to destination vector forming the expression clone. 2016 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91427/1/FP%202016%20124%20IR.pdf Yamba, Mohd Saiful (2016) Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm. [Project Paper Report] |
| institution |
UPM IR |
| collection |
UPM IR |
| language |
English |
| description |
The oil palm tissue contains vitamin E in the form of tocotrienols and
tocopherols. The combination of prenyldiphosphate and homogentisic acid (HGA) is the
first committed step in vitamin E production. The reaction is catalyzed by homogentisate
phytyltransferase (HPT). The oil palm genomic sequence containing the HPT gene is
used to study the expression of HPT gene in oil palm tissues. The objective of this study
is to isolate and clone the HPT promoter sequence. To obtain the result from this study,
some molecular work was done, including DNA extraction by DNA extraction kit and
the cetyltrimethylammonium bromide (CTAB) method, designing the primer for HPT
promoter isolation, running the polymerase chain reaction (PCR) to get the HPT
promoter sequence and performing agarose gel purification to purify the HPT promoter
sequences. After obtaining the amplified HPT promoter sequence, the HPT promoter
was introduced into an entry vector and then to destination vector forming the expression clone. |
| format |
Project Paper Report |
| author |
Yamba, Mohd Saiful |
| spellingShingle |
Yamba, Mohd Saiful Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm |
| author_facet |
Yamba, Mohd Saiful |
| author_sort |
Yamba, Mohd Saiful |
| title |
Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm |
| title_short |
Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm |
| title_full |
Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm |
| title_fullStr |
Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm |
| title_full_unstemmed |
Isolation and cloning of the promoter sequence of Vitamin E Biosynthesis gene (Homogentisate phytyltransferase) from oil palm |
| title_sort |
isolation and cloning of the promoter sequence of vitamin e biosynthesis gene (homogentisate phytyltransferase) from oil palm |
| publishDate |
2016 |
| url |
http://psasir.upm.edu.my/id/eprint/91427/1/FP%202016%20124%20IR.pdf |
| _version_ |
1819300508230221824 |
| score |
13.4562235 |