Plant regeneration from cotyledon and hypocotyl explants of red cabbage (Brassica oleracea var. capitata f. rubra) /
A study was carried out to determine the most suitable concentration of BAP on inducing multiple shoot regeneration from cotyledon and hypocotyl explant of Brassica oleracea var. capitata f.rubra (Red cabbage) seedlings germinated in vitro. A study was also carried out to determine the most suitable...
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2009.
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| Summary: | A study was carried out to determine the most suitable concentration of BAP on inducing multiple shoot regeneration from cotyledon and hypocotyl explant of Brassica oleracea var. capitata f.rubra (Red cabbage) seedlings germinated in vitro. A study was also carried out to determine the most suitable concentration of IBA on inducing roots from shoots of Brassica oleracea var. capilata f.rubra (Red cabbage). Experiment 1 was performed using cotyledon as explants for shoot regeneration. There were six treatments tested consisting of BAP at the concentration of 0, 1, 1.5, 2, 2.5, and 3 mg/L. All the treatments resulted in shoot regeneration with the highest at 46.70% obtained on medium with 1.5 mg/L BAP (T3). Meanwhile, medium without BAP (Tl-control) showed the highest percentage of callus formation at 66.67%. Experiment 2 was performed using hypocotyls as explants for shoot regeneration. There were six treatments consisting of BAP at 0, 1, 2.5, 3, 3.5, and 4 mg/L. Overall, all the treatments produced shoots. The most suitable BAP concentration was 3mg/L (T4) which produced 53.33% of shoot formation. Observation on callus formation showed T3 and T5 (2.5 and 3.5mg/L BAP) producing the highest percentage of callus formation (13.33%). Experiment 3 was performed on rooting of shoots. There were six treatments consisting of IBA at the concentration of 0, 0.1, 0.5, 1, 1.5, and 2 mg/L. The most suitable IBA concentration was 0.5mg/L (T3) which produced 53.33% of explant with root formation. While, the lowest percentage of explant with roots (20.00%) was obtained on IBA at 2mg/L (T6). |
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| Physical Description: | 48 leaves : ill. ; 30cm. |