Effect of plant growth promoting Rhizobacteria on growth of misai kucing (Orthosiphon stamineus) /
Herbs have been used as food and medicine for centuries. Misai kucing (Orthosiphon stamineus) is one of the 6000 tropical species that has been reported to possess medicinal values and useful in cancer treatments of various diseases especially. Plant Growth Promoting Rhizobacteria (PGPR) are being u...
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012.
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| Summary: | Herbs have been used as food and medicine for centuries. Misai kucing (Orthosiphon stamineus) is one of the 6000 tropical species that has been reported to possess medicinal values and useful in cancer treatments of various diseases especially. Plant Growth Promoting Rhizobacteria (PGPR) are being used as biohancer and biofertilizer for many herbal crops. Inoculation with PGPR benefits the host plant by improving root development and increasing the biomass, yield and N content. Therefore, the aims of this research were to isolate and screen for rhizobacteria from surface roots (rhizosphere and rhizoplane) and internal roots (endopyhte) of Misai Kucing (Orthosiphon stamineus) and to screen the ability of rhizospheric and endophytic isolates to produce indole-3-acetic acid (IAA) and fix atmospheric nitrogen. The study also determined the effect of locally isolated PGPR (Misai Kucing) and UPMB-IO on vegetative growth (roots and tops) of Misai Kucing. Nine treatments [TO: -I-N+F ( uninoculated, no nitrogen control, with P and K fertilizer), TI: -I + 30% N + F (uninoculated, 30% nitrogen control, with P and K fertilizer), T2: -1+100% N+F (uninoculated, 100% nitrogen control, with P and K fertilizer), T3:(+ PGPR UPMB-IO)-N+F (inoculated, no nitrogen, with P and K fertilizer), T4: (+ PGPR from Misai Kucing I, ARl)-N+F (inoculated, no nitrogen, with P and K fertilizer), T5: (+ PGPR from Misai Kucing 2, AR2)-N+F (inoculated, no nitrogen, with P and K fertilizer), T6:(+ PGPR UPMB-IO)+30%N+F (inoculated, 30% nitrogen, with P and K fertilizer), T7: (+ PGPR from Misai Kucing I, ARI)+30% N + F (inoculated, 30% nitrogen, with P and K fertilizer), T8: (+ PGPR from Misai Kucing 2, AR2)+30% N + F (inoculated, 30% nitrogen control, with P and K fertilizer) ]. Plants were sampled at 50 days of growth. All data collected were analyzed using Analysis of Variance (ANOVA) while means of data were compared using T test in the SAS. Experimental design was RCBD with 5 replications. The results showed that the best two PGPR which increased the plant growth as measured by plant height, plant fresh and dry weight and content of nitrogen were T3:(+ PGPR UPMB-IO)-N+F (inoculated, no nitrogen, with P and K fertilizer) and T6:(+ PGPR UPMB-IO)+30%N+F (inoculated, 30% nitrogen, with P and K fertilizer). In conclusion, inoculation of locally isolated PGPR and UPMB-IO on Misai Kucing will promote the plant growth significantly compared to uninoculated Misai Kucing. |
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| Physical Description: | 57 leaves : ill. ; 30cm. |