Serological andmolecular prevalence of ehrlichia canis in stray dogs in Kuala Lumpur /
A molecular and serological prevalence study of E.canis in stray dogs in Kuala Lumpur was carried out. Fifty eight stray dogs from Dewan Bandaraya Kuala Lumpur (DNKL) dog proud were included in this study. PCR was carried out on both blood and splenic aspirate samples using E.canis species – speci...
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| Format: | Thesis Book |
| Language: | English |
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2013
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| LEADER | 01704nam a2200217 a 4500 | ||
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| 001 | vtls027076314 | ||
| 003 | UPM | ||
| 005 | 20161020195549.0 | ||
| 008 | 130528s2012 my a b 000 0 eng d | ||
| 039 | 9 | |y 201305281518 |z harina | |
| 040 | |c UPM | ||
| 090 | 0 | 0 | |a FPV 2013 29 |
| 100 | 1 | |a Yee Jia Yin | |
| 245 | 1 | 0 | |a Serological andmolecular prevalence of ehrlichia canis in stray dogs in Kuala Lumpur / |c Yee Jia Yin. |
| 260 | |a 2013 | ||
| 300 | |a 30 leaves : |b ill. ; |c 30cm. | ||
| 502 | |a Project paper (D.V.M) - Universiti Putra Malaysia, 2013. | ||
| 520 | |a A molecular and serological prevalence study of E.canis in stray dogs in Kuala Lumpur was carried out. Fifty eight stray dogs from Dewan Bandaraya Kuala Lumpur (DNKL) dog proud were included in this study. PCR was carried out on both blood and splenic aspirate samples using E.canis species – specific primers, CANS and GAIUR, that amplify a 409 bp fragment of the E.canis 16S rRNA gene. The serological prevalence of E.canis in stray dogs in Kuala Lumpur was determined using Biogal ImmunoComb® Canine Ehrlichia Antibody Test Kit. One out of fifty eight dogs (1.72%) was tested positive for E.canis via PCR in stray dogs in Kuala Lumpur was 1.72%. Whereas, 37 out of 58 dogs were seropositive using ImmunoComb® Canine Ehrlichia Antibody Test Kit. The serological prevalence was therefore 63.7%. All of the dogs were negative for E.canis under light mocroscopic examination. This is the first time splenic aspirate PCR to detect E.canis was attempted in Malaysia. | ||
| 942 | |2 lcc |c 40008 | ||
| 999 | |c 51490 |d 51490 | ||
| 952 | |0 0 |1 0 |4 0 |6 FPV2013 00029 |7 0 |9 56709 |a 40000 |b 40000 |c 40008 |d 2016-10-20 |o FPV 2013 29 |p 1000689425 |r 2016-10-20 |w 2016-10-20 |y 40008 | ||