Washroom contamination by HPV DNA and viability of HPV cells on non-living surface /
Human Papillomavirus (HPV) is a group of about 100 different types of DNA viruses that share some common genomic sequences. HPV infects the basal cell of squamous epithelium and is divided into cutaneous and mucosal type. Genital HPV especially HPV types -16 and -18 which belong to mucosal type are...
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| Format: | Thesis Book |
| Language: | English |
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2011.
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| 090 | 0 | 0 | |a FPSK2 2011 51 |
| 100 | 1 | 0 | |a Wong, Suk Chie. |
| 245 | 1 | 0 | |a Washroom contamination by HPV DNA and viability of HPV cells on non-living surface / |c Wong Suk Chie. |
| 246 | 3 | 0 | |a Pencemaran tandas awam oleh HPV DNA dan kadar penghidupan sel-sel HPV pada permukaan persekitaran. |
| 260 | 0 | 0 | |a 2011. |
| 300 | 0 | 0 | |a 68 leaves : |b ill. ; |c 30cm. |
| 502 | 0 | 0 | |a Project paper (B.S.(Biomedical Sciences)) - Universiti Putra Malaysia, 2011. |
| 520 | 0 | 0 | |a Human Papillomavirus (HPV) is a group of about 100 different types of DNA viruses that share some common genomic sequences. HPV infects the basal cell of squamous epithelium and is divided into cutaneous and mucosal type. Genital HPV especially HPV types -16 and -18 which belong to mucosal type are high risk viruses as they are related to 99.7% of cervical cancer cases. Therefore, studies on contamination of environmental surface by HPV and retention ofHPV DNA on non¬living surfaces are important for the prevention of HPV infection. This study is based on the hypothesis that HPV DNA is present in public washrooms; HPV cells can survive on non-living surface and HPV DNA can be stably retained on non¬living surfaces within a certain short period of time. For the first part of the study, 100 samples were collected from public toilets; subjected to DNA extraction and quantitation; ~-globin PCR and nested PCR followed by automated DNA sequencing and sequence analysis. In the second part of the study, 1 X 105 cultured HeLa cells was prepared in Roswell Park Memorial Institute (RPMI) media and phosphate buffered saline (PBS) respectively. One droplet of each cell suspension was placed onto non-living surfaces. Both droplets were tested with XTT assay to determine the viability of cells after 5, 10, 30, 60, 120 and 240 minutes. Simultaneously, another droplet of cell in RPMI media was collected by cotton swab and tested with nested PCR. Results showed that 28.5% of samples collected from public toilets had HPV DNA; XTT assays showed 77.5% ofRPMI-suspended HPV cells and 22.9% ofPBS¬suspended HPV cells can survive on plastic surface up to 120 minutes as compared to metal surface (RPMI: 5.24%; PBS: 7.72%) and tile surface (RPMI: 3.36%; PBS: 10.3%); and HPV DNA can stably retain on non-living surfaces up to 240 minutes. |
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