Sub-acute toxicity study of Dichloromethane extract of Dillenia suffruticosa in BALB/c mice /
The use of plants-derived products had increased significantly for the past few decades. Currently, it is estimated that about 80% of the world's population are still relying on herbal medicines for their healthneeds, due to the cost of orthodox treatment and medicines along with their long ter...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2016.
|
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The use of plants-derived products had increased significantly for the past few decades. Currently, it is estimated that about 80% of the world's population are still relying on herbal medicines for their healthneeds, due to the cost of orthodox treatment and medicines along with their long term side effects that substantially affect the quality of patient life. Although the use of herbal medicines is extensively practiced with the view that they are safe and effective, there are reports claim that herbs may also have adverse effects and lead to toxicity. Thus, it is a need for toxicity testing to evaluate the risk associated with the use of herbs, therefore avoiding potential harmful effects to the users. In recent years, the phytochemicals present in plants have received attention for development of agents to suppress the progression of cancer. One of the herbal plants with various medicinal values is Dillenia suffruticosath at locally known as Simpoh air. Dichloromethane extract of D. suffruticosa (DCM-DS) exhibited cytotoxicity through induction of apoptosis and cell cycle arrest in breast cancer cell lines. To date, there is yet any study of sub-acute toxicity of DCM-DS. The objective of the study was to investigate the sub-acute toxicity of DCM-DS in BALB/c mice. Root powder of D. slrffrrrticosa was supplied by Primer Herber Sdn. Bhd. Sequential solvent extraction was performed by using various solvents of increasing polarity (hexane and dichloromethane) to obtain DCM-DS. A total of thirty female BALB/c mice (6-8 weeks of age) was used. The mice were randomly assigned into five groups (n=6), which were (1) normal control group (distilled water), (2) negative control group (vehicle; 10% DMSO), and (3) three treatment groups of DCM-DS (200 mg/kg, 100 mg/kg and 50 mg/kg, by oral gavage daily). Duration of the study was 28 days. Throughout the treatment period, the animals were observed daily (before and after dosing) for general physical conditions such as appearance, behavior, toxicity symptoms and mortality. The body weight of the mice was measured twice weekly. After 28 days, all mice were anaesthetized and blood samples were collected by cardiac puncture for hematological (RBC, WBC and Hb) and biochemical analyses (liver marker enzymes: ALT, AST, ALT; and kidney functions: urea and creatinine). The kidney, heart, lung, spleen and liver of the mice were excised and fixed in 10% buffered formalin for histological analysis. Data were analyzed by analysis of variance (ANOVA). Data were expressed as mean ± standard error of mean (SEM) with significant level of p<0.05. There was no treatment-related mortality of mice at any dose level of DCM-DS tested. All the animals treated with DCM-DS at any dose level did not show any symptoms of toxicity. The changes in body weight, percentage of organs to body weight ratio and hematological parameters were not significant (p>0.05) for all the treatment groups compared to the control. For biochemical parameters, all were in the normal range except for ALP. Histology of liver and kidney tissue also revealed no abnormality. In conclusion, DCM-DS did not exert sub-acute toxicity to the BALB/c mice during the treatment period of 28 days. |
|---|---|
| Physical Description: | 56 leaves : ill. ; 30cm. |