Phylogenetic relationship between selected Aquilaria species based on the psbC-trnS region of the chloroplast DNA /
Agarwood producing species belonging to the Aquilaria genus is a highly endangered tree due to its substantial economic value. There are about 20 species in the genus. Species identification is difficult due to the high similarity in vegetative morphology. Therefore, method like DNA sequencing offer...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2015.
|
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Agarwood producing species belonging to the Aquilaria genus is a highly endangered tree due to its substantial economic value. There are about 20 species in the genus. Species identification is difficult due to the high similarity in vegetative morphology. Therefore, method like DNA sequencing offers an attractive alternative. Past studies have shown that the chloroplast DNA (cpDNA) can be used to investigate phylogenetic relationship at a wide range of taxonomic levels in plants. In this study, the feasibility of the psbC-trnS region was tested to differentiate 11 of Aquilaria species. Genomic DNA was extracted from young leaves or shoots using the DNeasy Plant Mini Kit. PCR amplification was carried out using two sets of primers, CS1 and CS2, and the PCR products were directly sequenced. Sequences from the noncoding cpDNA between psbC and trnS were examined and phylogenetic trees were constructed. Species relatedness between the species was tested by performing phylogenetic analysis using the Neighbor-Joining (NJ) programme. The psbC-trnS region provides variable and phylogenetically informative sites and resolved tree with supported clades. However, the CS1 primer set appeared to resolve better species clustery when compared to CS2. In conclusion, more regions should be tested in order to find several that could be used to establish species relatedness in Aquilaria. |
|---|---|
| Physical Description: | 50 leaves : ill. ; 30cm. |