Lipid signalling pathway gene expression in hep-g2 cells supplemented with exogenous lipids and stevia extract /
Dyslipidaemia such as hypertrigly cerida emia or hypercholesterolaemia can lead to atherosclerosis. Although simvastatin has been used widely to control hypercholesterolaernia in human, it is of interest to seek alt ernati ve treatment, especially in the veterinal)' field. Stevia (Stevia rebaud...
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| Materialtyp: | Lärdomsprov Bok |
| Språk: | English |
| Publicerad: |
2019.
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| Sammanfattning: | Dyslipidaemia such as hypertrigly cerida emia or hypercholesterolaemia can lead to atherosclerosis. Although simvastatin has been used widely to control hypercholesterolaernia in human, it is of interest to seek alt ernati ve treatment, especially in the veterinal)' field. Stevia (Stevia rebaudiana ) was found to have a hypolipidaemic effect, thus we hypothesized that cells supplemented with stevia extract will not express the same lipid signalling pathway gene as cells supplemented with only exogenous lipid . This study has been designed to observe the effect of stevia on mammalian cell lines (Hep-G2) supplemented with commercial stevia extract and stevia derived glycosides such as stevioside or Rebaudioside A The expression of genes involved in lipid signalling pathway such as low-density lipoprotein receptor (LDLr), 3-hydroxyl- 3methylglutaryl-coenzyme A reductase (HMGCR), scavenger receptor class b type 1 (SCAR-B l ), acyl-coenzyme A:cholesterol acyltransferase (ACAT2), perilipin 2 (PLIN2), proprotein convertase subtilisin/kexin type 9 (PCSK9) were assessed using gel electrophoresis after RT-PCR was performed. Glyceraldeh yde-3 phosphate dehydrogenase (GAPDH) was chosen as a housekeeping gene. LDLr were quantified using RT-qPCR and significantly showed an increase of 10.8, 14.0, 19.1 fold respectively in th positive control, high dose commercial stevia, and high dose stevioside . |
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| Fysisk beskrivning: | 32 leaves : ill. ; 30cm. |