Prevalence of bacteria in marine food fishes cultured in floating net-cages at Pulau Ketam, Selangor, Malaysia /

Infection by numerous bacterial diseases in marine fishes can cause economic losses in the mariculture sector. Therefore this study was conducted with three main goals i.e. i) to isolate and identify bacteria pecies in marine cage-culture at Pulau Ketam Selangor, ii) to determine bacterial loads of...

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Bibliographic Details
Main Author: Nurul Aqilah Mohd Soib, (Author)
Format: Thesis Book
Language:English
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040 |c UPM 
090 |a FPV 2020 2 
100 1 |a Nurul Aqilah Mohd Soib,  |e author. 
245 1 0 |a Prevalence of bacteria in marine food fishes cultured in floating net-cages at Pulau Ketam, Selangor, Malaysia /  |c Nurul Aqilah Binti Mohd Soib. 
264 0 |c 2020 
300 |a 36 leaves :  |b illustrations ;  |c 30cm. 
336 |2 rdacontent  |a text 
337 |2 rdamedia  |a unmediated 
338 |2 rdacarrier  |a volume 
502 |a Project paper (D.V.M) - Universiti Putra Malaysia, 2020. 
520 |a Infection by numerous bacterial diseases in marine fishes can cause economic losses in the mariculture sector. Therefore this study was conducted with three main goals i.e. i) to isolate and identify bacteria pecies in marine cage-culture at Pulau Ketam Selangor, ii) to determine bacterial loads of marine aquatic bacteria species (cfu/g or cfu/mL) in selected marine fish's organs, iii) to determine the prevalenc of the bact rial agent infecting cultured marine fi hes in a grow-out cage culture farm Pulau Ketam langor Malay ia. In thi study, a total of ten marine fishes of mixed-species which consi ted of three tails of grouper (Epinephelus sp.) and seven tai s of snapper (L,,(janit, p.) were collect d. The samples were t ntatively group d into clinically infected fish (Group A) and apparently healthy fish (Group B). Organ's inocula from kidney, liver, intestine, and abnormal lesion were overlaid onto Thiosulfate-citrate-bile salts-sucrose agar (TCBS) and marine Trypticase soy agar (TSA added with 1% sodium chloride). Biochemical tests and API 20E done showed that there were several marine bacterial species isolated and identified, namely, Photobacterium damselae, Burkholderia cepacia, and Aeromonas hydrophila. Results revealed that the highest prevalence of bacterial isolates in san1pled fish was Photobacterium damselae (39.29%), followed by Burkholderia cepacia (35.71%) and Aeromonas hydrophila (25.0%). Prevalence of Ph. damselae was recorded highest in intestine (18.18%) in group A and in kidney (27.27%) in Group B. Burkholderia cepacia was mainly found in intestine of both groups, group A (20.00%) and Group B (50.00%). Aeromonas hydrophila was recorded highest in intestine and kidney in Group A (14.29%) and in intestine in Group B (42.86%). The total aerobic colony-forming unit (CFU) for kidney, and liver in Group A was higher than in the sampled organs in Group B, which could be explained by high bacterial loads in organs of Group A as a result of bacterial infection. Bacteria species isolated were potential pathogen infecting marine fishes at Pulau Ketam, Selangor.  
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