Genetic diversity and protein profiling of selected freshwater fish /
Molecular biology has unlocked secrets of mystifying new disease, given us the premier tools for defining biological identity and created a pillar of data to support Darwin’s theory of common descent. In short, molecular biology and its elegant techniques have revolutionized our understanding of lif...
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2011.
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| Summary: | Molecular biology has unlocked secrets of mystifying new disease, given us the premier tools for defining biological identity and created a pillar of data to support Darwin’s theory of common descent. In short, molecular biology and its elegant techniques have revolutionized our understanding of life’s origins and mechanisms. Furthermore, fish protein is one of the important protein sources. Thus, six types of common freshwater fish was selected which are Tilapia Merah (Oreochromis sp. Red Tilapia), Tilapia Hitam (Oreochromis mossambicus), Catfish (Ictalurus punctatus), Silver Catfish (Pangasius pangasius), Snakehead Fish (Channa striata) and Silver Barb (Barbonymus gonionotus). Freshwater fish are fish that lives in freshwater, such as rivers and lakes, with a salinity of less than 0.05%. The type of fish species were selected based on the criteria of easily accessible and commonly found. Besides, these fishes are also economically important food that has gained popularity among consumers due to its high nutritional value especially protein and good taste. The protein profiling allows researchers to employ protein electrophoresis as the most widely used technique in life science research to study protein structure and function. The purpose of using SDS-PAGE is to generate protein profiles from the muscle both distantly and closely related species of fish. Results obtained from the reported literature concluded that protein profiles can be used as indicators of evolutionary relatedness. This study is conducted to measure the differences and similarity aspects of fish muscle protein can be determined. The general objective is characterization of fish muscle protein and genetic diversity of selected fresh water fish. There are two specific objectives which are to characterize muscle protein of selected fresh water fish and to compare phyogenetic trees of 16s RDNA generated. Crude muscle from each type of fishes was extracted with common procedure and further resolved using Sodium Dodecyl Sulphate Polycrylamide Gel Electrophoresis (SDS-PAGE). Banding profiles generated were subjected to cluster analysis with Pearson coefficient. Further analysis of genetic diversity utilizes 16— rDNA and pairwise alignments were conducted on genetic sequences obtained from Genbank. The relatedness based on protein profile was compared with the relatedness of fishes obtained from 16— rDNA sequences alignment. The highest percentage of similarity on protein profile is belongs to Tilapia Hitam and Tilapia Merah which is less or equivalent with 85% similarity. On the other hand, Haruan and Patin exhibited less than 84% similarity. The similarity between these two clusters i.e. Haruan, Patin and Tilapia Merah, Tilapia Hitam were less than 78%. Furthermore, Keli possess 67% similarity with Haruan, Patin, Tilapia Hitam and Tilapia Merah according to the protein profile obtained. Finally, Lampam Jawa revealed less than 60% similarity with Keli, Patin, Haruan, Tilapia Merah and Tilapia Hitam. The discovery of protein relatedness profile will allow researcher to further identify new species or closest related species with the beneficial component of fishes. Since Haruan already is well known for its medical benefit such as wound healing properties, identification of fishes closely related with this fish especially on the protein component will enable the discovery this benefit on other fishes. Thus, Patin can be the alternative source that might possesses wound healing properties because analysis from the protein profiling indicated Patin is closely related with Haruan. |
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| Physical Description: | 45 leaves : ill. ; 30cm. |