Structural Genomics and Drug Discovery Methods and Protocols /
Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2014.
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| Edition: | 1st ed. 2014. |
| Series: | Methods in Molecular Biology,
1140 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-0354-2 |
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Table of Contents:
- Data Management in the Modern Structural Biology and Biomedical Research Environment
- Structural Genomics of Human Proteins
- Target Selection for Structural Genomics of Infectious Diseases
- Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery
- High Throughput Cloning for Biophysical Applications
- Expression and Solubility Testing in a High Throughput Environment
- Protein Production for Structural Genomics Using E. coli Expression
- Eukaryotic Expression Systems for Structural Studies
- Automated Cell-free Protein Production Methods for Structural Studies
- Parallel Protein Purification
- Oxidative Refolding from Inclusion Bodies
- High throughput Crystallization Screening
- Screening Proteins for NMR Suitability
- Salvage or Recovery of Failed Targets by in situ Proteolysis
- Salvage of Failed Protein Targets by Reductive Alkylation
- Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy
- Data Collection for Crystallographic Structure Determination
- Structure, Determination, Refinement, and Validation
- Virtual High-Throughput Ligand Screening
- Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)
- Ligand Screening using Enzymatic Assays
- Ligand Screening using NMR
- Screening Ligands by X-ray Crystallography
- Case Study – Structural Genomics and Human Protein Kinases.