Plant Circadian Networks Methods and Protocols /
Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vi...
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| Další autoři: | |
| Médium: | Elektronický zdroj E-kniha |
| Jazyk: | English |
| Vydáno: |
New York, NY :
Springer New York : Imprint: Humana,
2014.
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| Vydání: | 1st ed. 2014. |
| Edice: | Methods in Molecular Biology,
1158 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-4939-0700-7 |
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Obsah:
- Measurement of Luciferase Rhythms
- Online Period Estimation and Determination of Rhythmicity in Circadian Data, using the BioDare Data Infrastructure
- Global profiling of the circadian transcriptome using microarrays
- ChIP-Seq analysis of histone modifications at the core of the Arabidopsis circadian clock
- Quantitative transcriptome analysis using RNA-seq
- Rapid and parallel quantification of small and large RNA species
- The RIP per case – identification of RNA binding protein targets by RNA immunoprecipitation
- A protocol for visual analysis of alternative splicing in RNA-Seq data using Integrated Genome Browser
- AthaMap web tools for the analysis of transcriptional and post-transcriptional gene expression regulation in Arabidopsis thaliana
- Analysis of mRNA translation states in Arabidopsis over the diurnal cycle by polysome microarray
- Immunoprecipitation-based analysis of protein-protein interactions
- Comparative Phosphoproteomics to Identify Targets of the Clock-Relevant Casein Kinase 1 in C. reinhardtii Flagella
- Pulsed induction of circadian clock genes in Arabidopsis seedlings
- The use of fluorescent proteins to analyze circadian rhythms
- Measuring circadian oscillations of cytosolic-free calcium in Arabidopsis thaliana
- Circadian life without micronutrients: effects of altered micronutrient supply on clock function in Arabidopsis
- Assessing redox state and reactive oxygen species in circadian rhythmicity
- Circadian regulation of plant immunity to pathogens
- Determination of photoperiodic flowering time control in Arabidopsis and barley
- The perennial clock is an essential timer for seasonal growth events and cold hardiness
- Monitoring seasonal bud set, bud burst and cold hardiness in Populus
- Transformation and Measurement of Bioluminescence Rhythms in the Moss Physcomitrella patens
- Modeling and Simulating the Arabidopsis thaliana Circadian Clock Using XPP-AUTO.